Overview

  • Product nameAnti-psbA2 antibody
  • Description
    Rabbit polyclonal to psbA2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana, Tobacco
    Predicted to work with: Plants
  • Immunogen

    Synthetic peptide:

    PVDI DGIREPVAGS LLYGNNIISG AVVPS

    , corresponding to amino acids 57-85 of Synechocystis sp. PCC 6803 psbA2 (NP_439906.1). The peptide is conserved in D1 protein sequences from all photosynthetic organisms

  • Positive control
    • Arabidopsis and tobacco chloroplast, and Synechocystis thylakoid proteins.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight of precursor: 39 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevancepsbA2, also known as psbA2 photosystem II D1 protein, is one the major proteins of the reaction centre of photosystem II which carries the QB-binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane.
    • Cellular localizationCellular thylakoid membrane; Multi-pass membrane protein
    • Database links
      • Alternative names
        • 32 kDa thylakoid membrane protein 2 antibody
        • Photosystem II protein D1 2 antibody
        • Photosystem Q(B) protein 2 antibody
        • psbA 2 antibody
        • psbA2 antibody
        • psbA2 photosystem II D1 protein antibody
        • psbA3 antibody
        see all

      Anti-psbA2 antibody images

      • All lanes : Anti-psbA2 antibody (ab65578) at 1/1000 dilution

        Lane 1 : Arabidopsis chloroplast proteins
        Lane 2 : Tobacco chloroplast proteins
        Lane 3 : Synechocystis thylakoid proteins

        Developed using the ECL technique

        Predicted band size : 39 kDa
        Observed band size : 35 kDa (why is the actual band size different from the predicted?)

      References for Anti-psbA2 antibody (ab65578)

      ab65578 has not yet been referenced specifically in any publications.

      Product Wall

      Application Western blot
      Loading amount 25 µg
      Gel Running Conditions Reduced Denaturing (12% TGX Bio-Rad Gel)
      Sample Chlamydomonas reinhardtii Cell lysate - whole cell (C. reinhardtii lysate)
      Specification C. reinhardtii lysate
      Treatment 2 M Urea for 1 hr on ice + loading buffer
      Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Username

      Grad Student. Javier Gimpel

      Verified customer

      Submitted Apr 04 2014

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"