Overview

  • Product nameAnti-psbD antibody
  • Description
    Rabbit polyclonal to psbD
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana, Rice
    Predicted to work with: Plants
  • Immunogen

    Synthetic peptide:

    ANTFRAFNPTQ

    , corresponding to amino acids 230-240 of Arabidopsis thaliana psbD

  • Positive control
    • Spinach and Arabidopsis thylakoids.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 39 kDa).

Target

  • RelevancepsbD (D2) is one of the two reaction center proteins of PSII. D2 protein is needed for assembly of a stable PSII complex and bear two tyrosine donors which are placed at a luminal loop of D1 and D2. D2 polypeptide has five transmembrane helices. In both plants and cyanobacteria, the hydrophobic polypeptides D1 and D2, bind most of the prosthetic groups that are involved in electron transport.
  • Cellular localizationPlastid, chloroplast thylakoid membrane; Multi-pass membrane protein.
  • Database links
    • Alternative names
      • D2 protein antibody
      • Photosystem II D2 protein antibody
      • Photosystem Q(A) protein antibody
      • psbD photosystem II protein D2 antibody
      • PSII D2 protein antibody
      see all

    Anti-psbD antibody images

    • All lanes : Anti-psbD antibody (ab65392) at 1/2000 dilution

      Lane 1 : Spinach thylakoids
      Lane 2 : Arabidopsis thylakoids


      Predicted band size : 39 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-psbD antibody (ab65392)

    ab65392 has not yet been referenced specifically in any publications.

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