Overview

  • Product nameAnti-PSCA antibody
    See all PSCA primary antibodies
  • Description
    Mouse monoclonal to PSCA
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: CYSCKAQVSN EDCLQVENCT QLGEQCWTAR IRAVGLLTVI SKGCSLNCVD DSQDYYVGKK NITCCDTDLC NAS, corresponding to amino acids 23-96 of Human PSCA

Properties

Applications

Our Abpromise guarantee covers the use of ab56338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • RelevancePSCA (Prostate Stem Cell antigen) is a cell surface antigen, which is overexpressed in ~40% of primary prostate cancers and in as many as 100% of metastatic ones. PSCA is also overexpressed in a majority of transitional cell and pancreatic carcinomas. Antibody directed against PSCA inhibits tumorigenesis, slows tumor growth, prolongs survival and prevents metastasis in a preclinical zenograft model indicating that PSCA may have utility as a prognostic marker and/or therapeutic target in prostate cancer.
  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor.
  • Database links
  • Alternative names
    • PRO 232 antibody
    • PRO232 antibody
    • Prostate stem cell antigen antibody

Anti-PSCA antibody images

  • Western blot against tagged recombinant protein immunogen using ab56338 PSCA antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
  • IHC image of ab56338 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab56338, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-PSCA antibody (ab56338)

This product has been referenced in:
  • Liu N  et al. Simultaneous and combined detection of multiple tumor biomarkers for prostate cancer in human serum by suspension array technology. Biosens Bioelectron 47:92-8 (2013). Human . Read more (PubMed: 23567627) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Specification Prostate cancer
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Kai Safferling

Verified customer

Submitted Nov 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"