Overview

  • Product name
  • Description
    Goat polyclonal to PSCDBP
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: SLQRLLQHSSNGN, corresponding to N terminal amino acids 2-14 of Human PSCDBP.

  • Positive control
    • Jurkat lysate.
  • General notes
    GenBank Accession Number – NP_004279.

Properties

Applications

Our Abpromise guarantee covers the use of ab2247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).Can be blocked with Human PSCDBP peptide (ab22893).
ELISA 1/16000.

Target

  • Function
    By its binding to cytohesin-1 (CYTH1), it modifies activation of ARFs by CYTH1 and its precise function may be to sequester CYTH1 in the cytoplasm.
  • Tissue specificity
    Expressed in lymph nodes, thymus, spleen, lung, peripheral blood leukocytes and bone marrow.
  • Sequence similarities
    Contains 1 PDZ (DHR) domain.
  • Cellular localization
    Cytoplasm. Early endosome. Recruited from the cytosol to endosomes by SNX27.
  • Information by UniProt
  • Database links
  • Alternative names
    • B3-1 antibody
    • B31 antibody
    • CASP antibody
    • Cbp HE antibody
    • CYBR antibody
    • CYTHIP antibody
    • Cytip antibody
    • CYTIP_HUMAN antibody
    • Cytohesin 1 interacting protein antibody
    • Cytohesin binder and regulator antibody
    • Cytohesin-associated scaffolding protein antibody
    • Cytohesin-binding protein HE antibody
    • Cytohesin-interacting protein antibody
    • HE antibody
    • Pleckstrin Homology Sec7 and Coiled-Coil Domains Binding Protein antibody
    • Pleckstrin homology Sec7 and coiled-coil domains-binding protein antibody
    • Pleckstrin homology, Sec7 and coiled/coil domains, binding protein antibody
    see all

Anti-PSCDBP antibody images



  • Predicted band size : 41 kDa


    ab2247 staining (1 µg/ml) of MOLT4 cell lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

References for Anti-PSCDBP antibody (ab2247)

This product has been referenced in:
  • O'Brien M  et al. Upregulation of PSCDBP, TLR2, TWIST1, FLJ35382, EDNRB, and RGS12 gene expression in human myometrium at labor. Reprod Sci 15:382-93 (2008). Read more (PubMed: 18497345) »
  • MacNeil AJ  et al. Sorting nexin 27 interacts with the Cytohesin associated scaffolding protein (CASP) in lymphocytes. Biochem Biophys Res Commun 359:848-53 (2007). IP ; Human . Read more (PubMed: 17577583) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (AGS human gastric carcinoma cells)
Specification
AGS human gastric carcinoma cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Type
Sandwich (Detection)
Username

Abcam user community

Verified customer

Submitted Jul 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Loading amount
10 µg
Specification
AGS Gastric Carcinoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 17 2010

Thank you very much for your enquiry and patience. Below is the Western blotting protocol that the originator used with ab2247. I hope this helps and please let me know if you need further assistance. - Lysis. Cell pellets were washed with ice-cold ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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