Overview

  • Product nameAnti-PSD3 antibody
    See all PSD3 primary antibodies
  • Description
    Rabbit polyclonal to PSD3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: TSSNVEAAKR LAKRLYQLDR FKRSDVAKHL GKNNEFSKLV AEEYLKFFDF, corresponding to internal sequence amino acids 577-626 of Human PSD3

  • Positive control
    • A204 cell lysate, human skeletal muscle tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevancePSD3 is a guanine nucleotide exchange factor (GEF) specific for ARF6-like protein. The gene is potential tumour suppressor or antagonising gene candidate for ovarian carcinoma.
  • Cellular localizationCell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity).
  • Database links
  • Alternative names
    • ADP ribosylation factor guanine nucleotide factor 6 antibody
    • DKFZp761K1423 antibody
    • EFA 6R antibody
    • EFA6R antibody
    • Epididymis tissue protein Li 20mP antibody
    • Exchange factor for ADP ribosylation factor guanine nucleotide factor 6 antibody
    • HCA 67 antibody
    • HCA67 antibody
    • Hepatocellular carcinoma associated antigen 67 antibody
    • KIAA0942 antibody
    • PH and SEC7 domain containing protein 3 antibody
    • Pleckstrin and Sec7 domain containing 3 antibody
    • Pleckstrin and Sec7 domain containing protein 3 antibody
    • Pleckstrin homology and SEC7 domain containing protein 3 antibody
    • PSD 3 antibody
    • PSD3 protein antibody
    see all

Anti-PSD3 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling PSD3 with ab62194 at 4-8µg/ml. Arrows inidcate positively labelled skeletal muscle cells. Magnification: 400X.

  • Anti-PSD3 antibody (ab62194) at 2.5 µg/ml + A204 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-PSD3 antibody (ab62194)

ab62194 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. The protocols used was as below. 1. Deparaffinize sections in 3 changes of xylene, 5 minutes each 2. Hydrate in 2 changes of 100% ethanol for 3 minutes each, 95% and 80% ethanol for 1 minute each. ...

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