Overview

  • Product nameAnti-PSD3 antibody
    See all PSD3 primary antibodies
  • Description
    Rabbit polyclonal to PSD3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: TSSNVEAAKR LAKRLYQLDR FKRSDVAKHL GKNNEFSKLV AEEYLKFFDF, corresponding to internal sequence amino acids 577-626 of Human PSD3

  • Positive control
    • A204 cell lysate, human skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevancePSD3 is a guanine nucleotide exchange factor (GEF) specific for ARF6-like protein. The gene is potential tumour suppressor or antagonising gene candidate for ovarian carcinoma.
  • Cellular localizationCell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity).
  • Database links
  • Alternative names
    • ADP ribosylation factor guanine nucleotide factor 6 antibody
    • DKFZp761K1423 antibody
    • EFA 6R antibody
    • EFA6R antibody
    • Epididymis tissue protein Li 20mP antibody
    • Exchange factor for ADP ribosylation factor guanine nucleotide factor 6 antibody
    • HCA 67 antibody
    • HCA67 antibody
    • Hepatocellular carcinoma associated antigen 67 antibody
    • KIAA0942 antibody
    • PH and SEC7 domain containing protein 3 antibody
    • Pleckstrin and Sec7 domain containing 3 antibody
    • Pleckstrin and Sec7 domain containing protein 3 antibody
    • Pleckstrin homology and SEC7 domain containing protein 3 antibody
    • PSD 3 antibody
    • PSD3 protein antibody
    see all

Anti-PSD3 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling PSD3 with ab62194 at 4-8µg/ml. Arrows inidcate positively labelled skeletal muscle cells. Magnification: 400X.

  • Anti-PSD3 antibody (ab62194) at 2.5 µg/ml + A204 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-PSD3 antibody (ab62194)

ab62194 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The protocols used was as below. 1. Deparaffinize sections in 3 changes of xylene, 5 minutes each 2. Hydrate in 2 changes of 100% ethanol for 3 minutes each, 95% and 80% ethanol for 1 minute each. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"