Recombinant Anti-PSD93 antibody [EPR8740] (ab151721)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8740] to PSD93
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PSD93 antibody [EPR8740]
See all PSD93 primary antibodies -
Description
Rabbit monoclonal [EPR8740] to PSD93 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, SH-SY5Y, Jurkat and Human astrocytoma cell lysates; SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8740 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 97 kDa. |
Target
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Function
Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
Domain
An N-terminally truncated L27 domain is predicted in isoform 2 at positions 1 through 27. -
Post-translational
modificationsPalmitoylation of isoform 1 is not required for targeting to postsynaptic density. -
Cellular localization
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Membrane. Cell projection, axon. Concentrated in soma and postsynaptic density of a subset of neurons. - Information by UniProt
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Database links
- Entrez Gene: 1740 Human
- Entrez Gene: 23859 Mouse
- Entrez Gene: 64053 Rat
- Omim: 603583 Human
- SwissProt: Q15700 Human
- SwissProt: Q91XM9 Mouse
- SwissProt: Q63622 Rat
- Unigene: 367656 Human
see all -
Alternative names
- Channel associated protein of synapse 110 antibody
- Channel associated protein of synapses 110kD antibody
- Channel-associated protein of synapse-110 antibody
see all
Images
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All lanes : Anti-PSD93 antibody [EPR8740] (ab151721) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human astrocytoma cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 97 kDa -
Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labeling PSD93 with ab151721 at 1/100 dilution (red), compared to a nonspecific control antibody (green).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab151721 has been referenced in 2 publications.
- Liu FR et al. Pedigree-based study to identify GOLGB1 as a risk gene for bipolar disorder. Transl Psychiatry 12:390 (2022). PubMed: 36115840
- Zhang Q et al. PSD-93 mediates the crosstalk between neuron and microglia and facilitates acute ischemic stroke injury by binding to CX3CL1. J Neurochem 157:2145-2157 (2021). PubMed: 33599284