Recombinant
RabMAb

Anti-PSD93 antibody [EPR8740] (ab151721)

Overview

  • Product name
    Anti-PSD93 antibody [EPR8740]
    See all PSD93 primary antibodies
  • Description
    Rabbit monoclonal [EPR8740] to PSD93
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human PSD93 (UniProt: Q15700).

  • Positive control
    • HeLa, SH-SY5Y, Jurkat and Human astrocytoma cell lysates; SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses.
    • Sequence similarities
      Belongs to the MAGUK family.
      Contains 1 guanylate kinase-like domain.
      Contains 3 PDZ (DHR) domains.
      Contains 1 SH3 domain.
    • Domain
      An N-terminally truncated L27 domain is predicted in isoform 2 at positions 1 through 27.
    • Post-translational
      modifications
      Palmitoylation of isoform 1 is not required for targeting to postsynaptic density.
    • Cellular localization
      Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Membrane. Cell projection, axon. Concentrated in soma and postsynaptic density of a subset of neurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • Channel associated protein of synapse 110 antibody
      • Channel associated protein of synapses 110kD antibody
      • Channel-associated protein of synapse-110 antibody
      • Chapsyn 110 antibody
      • Chapsyn-110 antibody
      • Chapsyn110 antibody
      • Discs, large homolog 2 (Drosophila) antibody
      • Disks large homolog 2 antibody
      • DKFZp781D1854 antibody
      • DKFZp781E0954 antibody
      • Dlg 2 antibody
      • dlg2 antibody
      • DLG2_HUMAN antibody
      • Dlgh 2 antibody
      • Dlgh2 antibody
      • FLJ37266 antibody
      • Gm1197 antibody
      • MGC131811 antibody
      • Postsynaptic density protein PSD 93 antibody
      • Postsynaptic density protein PSD-93 antibody
      • Postsynaptic density protein PSD93 antibody
      • PSD 93 antibody
      • PSD93 antibody
      see all

    Images

    • All lanes : Anti-PSD93 antibody [EPR8740] (ab151721) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Human astrocytoma cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 97 kDa

    • Flow cytometric analysis of permeabilized SH-SY5Y cells labeling PSD93 with ab151721 at 1/100 dilution (red), compared to a nonspecific control antibody (green).

    References

    ab151721 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
    Specification
    Cryopreserved embryonic cortical neurons (QBMcells
    Permeabilization
    No
    Fixative
    paraformaldehyde with picric acid
    Username

    Ms. Babben Tinner

    Verified customer

    Submitted Jan 28 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina, plexiform layers)
    Specification
    Retina, plexiform layers
    Permeabilization
    Yes - Triton X
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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