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Western blot - PSD95 antibody [6G6-1C9] (ab2723)
Western blot - PSD95 antibody [6G6-1C9] (ab2723)
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Immunocytochemistry/ Immunofluorescence - PSD95 antibody [6G6-1C9] (ab2723)
Immunocytochemistry/ Immunofluorescence - PSD95 antibody [6G6-1C9] (ab2723)
(enlarge)


Product Name 

PSD95 antibody [6G6-1C9]

 

See all PSD95 antibodies (12)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [6G6-1C9] to PSD95

Immunogen 

Purified recombinant rat PSD-95.

Reacts with 
(species key)

Hu, Ms, Rat, Cow

Specificity 

Detects Post Synaptic Density 95 kDa (PSD-95) from rat tissues.

Tested applications 
(see key)

ICC/IF, IHC-Fr, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• ICC/IF

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• IF

1 Abreview

 

• IP

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• WB

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)


WB
(read Abreview)


ICC/IF
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
IF: 1/200.
IHC-Fr: 1/1000 (PMID 19038221).
WB: 1/2000. Detects a band of approximately ~95-105 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters

Relevance 

PSD95 is a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with DLG2. With DLG2 it is recruited into the same NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins.

 

Alternative names PSD95 antibody [6G6-1C9] (ab2723)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1742    (Human)
13385    (Mouse)
29495    (Rat)

GeneCard

    

GC17M007293    (Human)

Omim

    

602887    (Human)

SwissProt

    

P78352    (Human)
Q62108    (Mouse)
P31016    (Rat)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

6G6-1C9

Isotype 

IgG2a

Purity 

IgG fraction

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PSD95 antibody [6G6-1C9] is on this datasheet. But please do contact us if you would like any reassurance!


See below for PSD95 antibody [6G6-1C9] images, references, products related to ab2723 and other tools.

 

PSD95 antibody [6G6-1C9] images:

Product image  Western blot - PSD95 antibody [6G6-1C9] (ab2723)

Western blot - PSD95 antibody [6G6-1C9] (ab2723)

PSD95 antibody [6G6-1C9] (ab2723) at 0.0825 µg/ml + mouse forebrain at 20 µg

Observed band size : 95-105 kDa (why is the actual band size different from the predicted?)

Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

 

Product image  Immunocytochemistry/ Immunofluorescence - PSD95 antibody [6G6-1C9] (ab2723)

Immunocytochemistry/ Immunofluorescence - PSD95 antibody [6G6-1C9] (ab2723)

ab2723 at 1/200 staining rat primary hippocampal neurons by ICC/IF. The cells were paraformaldehyde fixed and then permeabilized with 0.1% Triton X-100 before being stained with the antibody for 1 hour. An Alexa-Fluor ® 594 conjugated goat polyclonal antibody was used as the secondary.

The antibody shows numerous puncta along the shafts.

This image is courtesy of an Abreview submitted by Dr Farida Korobova

See Abreview

 

References for PSD95 antibody [6G6-1C9] (ab2723)

Specific publication references   This product has been used in: (two most recent references)

Gonalves J et al. Methamphetamine-induced neuroinflammation and neuronal dysfunction in the mice hippocampus: preventive effect of indomethacin. Eur J Neurosci : (2010). WB; Mouse. PubMed: 20074221

Hoon M et al. Neuroligin 2 controls the maturation of GABAergic synapses and information processing in the retina. J Neurosci 29:8039-50 (2009). IHC-Fr; Mouse. PubMed: 19553444


See all 8 publication references for this product.


If you publish research using ab2723 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to PSD95

 

PSD95 antibody [6G6-1C9] - more information

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Other PSD95 Antibodies

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