![Western blot - PSD95 antibody [7E3-1B8] (ab13552) image](#ab13552.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody [7E3-1B8] (ab13552) image](#PSD95-Primary-antibodies-ab13552-1.jpg)
![Immunocytochemistry/ Immunofluorescence - PSD95 antibody [7E3-1B8] (ab13552) image](#PSD95-Primary-antibodies-ab13552-3.jpg)
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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Overview
Product name
Anti-PSD95 antibody [7E3-1B8]
See all PSD95 products (15) ...
Description
Mouse monoclonal [7E3-1B8] to PSD95
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow
Immunogen
Recombinant full length protein (Rat).
Positive control
Rat and mouse brain tissue extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
7E3-1B8
Isotype
IgG2a
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 100 kDa.
ICC/IF: Use at an assay dependent dilution.
Target
Function
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
Tissue specificity
Brain.
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Domain
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density.
Post-translational
modifications
Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Cell junction > synapse > synaptosome. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
Target information above from: UniProt accessionP78352
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
Database links
- Entrez Gene: 100137840 Cow
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- Unigene: 27256 Mouse
- Unigene: 9765 Rat
Anti-PSD95 antibody [7E3-1B8] images:
Western blot - PSD95 antibody [7E3-1B8] (ab13552)
![Western blot - PSD95 antibody [7E3-1B8] (ab13552)](/ps/datasheet/Images/13/ab13552/ab13552.jpg)
Western blot analysis of bovine brain tissue extract, probed with ab13552.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody [7E3-1B8] (ab13552)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody [7E3-1B8] (ab13552)](/ps/datasheet/images/13/ab13552/PSD95-Primary-antibodies-ab13552-1.jpg)
ab13552 at 1/100 dilution staining PSD95 in mouse backskin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. The image show basal, hair follicle and dermal staining.
Immunocytochemistry/ Immunofluorescence - PSD95 antibody [7E3-1B8] (ab13552)
![Immunocytochemistry/ Immunofluorescence - PSD95 antibody [7E3-1B8] (ab13552)](/ps/datasheet/images/13/ab13552/PSD95-Primary-antibodies-ab13552-3.jpg)
Central Panel: ab13552 staining PSD95 in rat primary hipoocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-BSA 3%) for 30 minutes at 20°C. An Cy5®-conjugated Donkey polyclonal to mouse IgG (dilution 1/500) was used as secondary antibody.
Left-hand panel: Homer GFP.
Right-hand panel: Merge
This image is courtesy of an anonymous Abreview
References for Anti-PSD95 antibody [7E3-1B8] (ab13552)
This product has been referenced in:
- Kruusmägi Met al. Functional differences between D(1) and D(5) revealed by high resolution imaging on live neurons. Neuroscience 164:463-9 (2009). WB; Rat.Read more (PubMed: 19723560) »
- Damiani Det al. Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina. J Neurosci 28:4878-87 (2008).Read more (PubMed: 18463241) »
See all 2 publications for this product
Publishing research using ab13552? Please let us know so that we can cite the reference in this datasheet
![PSD95 antibody [7E3-1B8] for ICC/IF in Rat (13552)](/ps/datasheet/images/13/ab13552/PSD95-Primary-antibodies-ab13552-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"