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Read our guarantee »Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Anti-PSD95 antibody [7E3-1B8]
See all PSD95 products (15) ...
Mouse monoclonal [7E3-1B8] to PSD95
WB, ICC/IFmore details
Reacts with
Mouse, Rat, Cow
Recombinant full length protein (Rat).
Rat and mouse brain tissue extract.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4
Concentration information loading...
Protein G purified
Monoclonal
7E3-1B8
IgG2a
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Our Abpromise guarantee covers the use of ab13552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 100 kDa.
ICC/IF: Use at an assay dependent dilution.
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
Brain.
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density.
Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
Cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Cell junction > synapse > synaptosome. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
Target information above from: UniProt accessionP78352
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - PSD95 antibody [7E3-1B8] (ab13552)
![Western blot - PSD95 antibody [7E3-1B8] (ab13552)](/ps/datasheet/Images/13/ab13552/ab13552.jpg)
Western blot analysis of bovine brain tissue extract, probed with ab13552.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody [7E3-1B8] (ab13552)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody [7E3-1B8] (ab13552)](/ps/datasheet/images/13/ab13552/PSD95-Primary-antibodies-ab13552-1.jpg)
ab13552 at 1/100 dilution staining PSD95 in mouse backskin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. The image show basal, hair follicle and dermal staining.
Immunocytochemistry/ Immunofluorescence - PSD95 antibody [7E3-1B8] (ab13552)
Central Panel: ab13552 staining PSD95 in rat primary hipoocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-BSA 3%) for 30 minutes at 20°C. An Cy5®-conjugated Donkey polyclonal to mouse IgG (dilution 1/500) was used as secondary antibody.
Left-hand panel: Homer GFP.
Right-hand panel: Merge
This image is courtesy of an anonymous Abreview
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab13552? Please let us know so that we can cite the reference in this datasheet
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![Western blot - PSD95 antibody [7E3-1B8] (ab13552)](/ps/datasheet/Images/13/ab13552/ab13552.jpg)
Western blot analysis of bovine brain tissue extract, probed with ab13552.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSD95 antibody [7E3-1B8] (ab13552)](/ps/datasheet/images/13/ab13552/PSD95-Primary-antibodies-ab13552-1.jpg)
ab13552 at 1/100 dilution staining PSD95 in mouse backskin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. The image show basal, hair follicle and dermal staining.
![Immunocytochemistry/ Immunofluorescence - PSD95 antibody [7E3-1B8] (ab13552)](/ps/datasheet/images/13/ab13552/PSD95-Primary-antibodies-ab13552-3.jpg)
Central Panel: ab13552 staining PSD95 in rat primary hipoocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-BSA 3%) for 30 minutes at 20°C. An Cy5®-conjugated Donkey polyclonal to mouse IgG (dilution 1/500) was used as secondary antibody.
Left-hand panel: Homer GFP.
Right-hand panel: Merge
This image is courtesy of an anonymous Abreview
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