Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab12093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionInteracts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • Tissue specificityBrain.
  • Sequence similaritiesBelongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • DomainThe PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • Post-translational
    modifications
    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • Cellular localizationCell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Anti-PSD95 antibody images



  • Performed under reducing conditions.

    Predicted band size : 105 kDa


    Exposure time : 10 seconds

    This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

    PSD95 (ab12093) western blot on mouse brain extract (10µg protein/lane), showing use of the immunising peptide (ab12419) as a control. The blot was exposed for 10 seconds to autoradiographic film.

    Lane 1 = 0.3µg ab12093
    Lane 2 = 1µg ab12093
    Lane 3 = 3µg ab12093
    Lane 4 = 3µg ab12093 + 1µg peptide
    Lane 5 = Ladder

  • ab12093 staining cultured rat primary hippocampal neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde and blocked with 10% donkey serum in  0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured neurons were then stained with ab12093 at 1/500 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-goat polyclonal antibody at 1/1000 was used as the secondary antibody. PSD95 expressed in exon, dendrites and particularly carrying in the dendritic spines

    See Abreview

  • All lanes : Anti-PSD95 antibody (ab12093) at 2 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 105 kDa
    Observed band size : 105 kDa
    Additional bands at : 30 kDa,35 kDa,70 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ab12093 PSD95 antibody immunostaining brain tissue (4% PFA perfused paraffin embedded 5 micron saggital section). ab12093 was used at 10 µg/ml for a 20 minute incubation at 37 degrees C (on a Ventana Discovery slide processor). Light antigen retrieval and then light protease treatment is recommended before addition of primary antibody ab12093 in this paradigm.
  • All lanes : Anti-PSD95 antibody (ab12093) at 1/5000 dilution

    Lane 1 : rat cortical lysate
    Lane 2 : mouse cortical lysate
    Lane 3 : BA21 human cortical lane lysate
    Lane 4 : BA21 human cortical lane lysate
    Lane 5 : BA21 human cortical lane lysate
    Lane 6 : BA21 human cortical lane lysate
    Lane 7 : BA21 human cortical lane lysate
    Lane 8 : BA21 human cortical lane lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexafluor 680

    Performed under reducing conditions.

    Predicted band size : 105 kDa

    This image is courtesy of an Abreview submitted by Dr Martin Broadstock

    See abreview for further details

    See Abreview

References for Anti-PSD95 antibody (ab12093)

This product has been referenced in:
  • Jara JH  et al. Healthy and diseased corticospinal motor neurons are selectively transduced upon direct AAV2-2 injection into the motor cortex. Gene Ther N/A:N/A (2016). ICC/IF ; Mouse . Read more (PubMed: 26704722) »
  • Pan W  et al. Effects of dihydrotestosterone on synaptic plasticity of the hippocampus in mild cognitive impairment male SAMP8 mice. Exp Ther Med 12:1455-1463 (2016). IHC-P ; Mouse . Read more (PubMed: 27588067) »

See all 14 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Zebrafish Tissue sections (Retina, plexiform layers)
Permeabilization Yes - triton X
Specification Retina, plexiform layers
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 05 2014

I am glad to read that most of the antibodies you ordered are giving good results. For the two that are not, could you please send some details of the samples and protocols and results? In particular, we would like to know the species, the time in PFA,...

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Thank you for contacting us. I want to apologize for the delay as we arranged to add each peptide to our catalog.

The blocking peptides which you have requested are now available and can be located at the following links:


<...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jun 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary Hippocampal neuron culture)
Specification Primary Hippocampal neuron culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Apr 16 2012

Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Coronal brain sections)
Specification Coronal brain sections
Username

Abcam user community

Verified customer

Submitted Oct 31 2011

Thank you for contacting us. We do not have anti GluR1 and GLuR2 antibodies that can be used for Zebrafish. We unfortunately do not have information of GluR1 and GLuR2 protein sequence of Zebrafish so I am sorry we can’t check the homology. How...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (BA21 human cortical tissue lysates)
Loading amount 20 µg
Specification BA21 human cortical tissue lysates
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Martin Broadstock

Verified customer

Submitted Apr 01 2009

Thank you for your rapid feedback, this information indeed helps. Could you please consider submitting a review when you have some time and in return we will offer you 50 Abcam Loyalty points which you can use to redeem a variety of gifts (another 1...

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Thank you for your e-mail, I am glad to hear you have solved your problem, would you mind letting me know what it was so I can close this enquiry? Thank you in advance,

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"