• Product name
    Anti-Pseudomonas fluorescens antibody
  • Description
    Goat polyclonal to Pseudomonas fluorescens
  • Specificity
    ab25812 recognises the majority of P. fluorescens proteins as seen by Silver Stain.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with Pseudomonas fluorescens. Not yet tested in other species.
  • Immunogen

    Tissue/ cell preparation: lysates of Pseudomonas fluorescens.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, HEPES (trace), Citric acid (trace). pH 7

    This antibody is provided in this buffer so the customer can perform additional modifications such as enzyme conjugation or biotinylation labeling without further processing or buffer exchange since the formulation contains no amines or other factors that might intefere in common conjugation chemistries.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was put through a Protein G IgG purification step prior to the antigen affinity purifcation step.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab25812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Note: ab25812 is useful as either capture and/or detection antibody for direct detection of antigen.
    WB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Pseudomonas fluorescens encompasses a diverse group of bacteria that is commonly found in a wide range of terrestrial and aquatic habitats. A strain that was naturally non-ice nucleating was originally isolated and registered in the USA for suppression of frost damage to fruit crops. Other strains with anti fungal or anti bacterial activity have been isolated and commercialized in the USA and India. Pseudomonas fluorescens is produced by fermentation. Its mode of action is as follows: bacteria and fungal propagules on the leaves of crops often serve as nucleation sites for ice formation and ice crystals often form when they are present and the temperature falls below freezing, with resulting damage to the leaf. If these are replaced on plant leaves with competitive antagonists (such as Pseudomonas fluorescens) that lack the ice nucleating protein, frost is prevented, even at temperatures as low as -5 °C. Other strains of Pseudomonos fluorescens are antagonistic to foliar or rhizosphere bacteria and fungi through the production of siderophores and antibiotics.

    References for Anti-Pseudomonas fluorescens antibody (ab25812)

    This product has been referenced in:
    • Arnold CP  et al. Pathogenic shifts in endogenous microbiota impede tissue regeneration via distinct activation of TAK1/MKK/p38. Elife 5:N/A (2016). IHC-P . Read more (PubMed: 27441386) »

    See 1 Publication for this product

    Product Wall

    Vielen Dank für Ihre Anfrage.
    Leider kann ich keine Anfrage von Jing Jin in unserem System finden. Ich bin mir nicht sicher dass die Antikörper ab25812 und ab37094 als Sonden in diesem Fall geeignet sind.
    Beide Antikörper sind für ELISA und W...

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