Recombinant
RabMAb

Anti-PSF1 antibody [EPR13359] (ab181112)

Overview

  • Product name
    Anti-PSF1 antibody [EPR13359]
    See all PSF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13359] to PSF1
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PSF1 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14691

  • Positive control
    • Jurkat, HeLa and HepG2 cell lysates; HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC/IF 1/250 - 1/500.
IP 1/50.

Target

  • Function
    The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS1 is essential for function.
  • Sequence similarities
    Belongs to the GINS1/PSF1 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA replication complex GINS protein PSF1 antibody
    • GINS complex subunit 1 (Psf1 homolog) antibody
    • GINS complex subunit 1 antibody
    • GINS1 antibody
    • partner of sld five-1 antibody
    • partner of sld5 antibody
    • PSF1 homolog antibody
    • PSF1_HUMAN antibody
    • RP4-691N24.2 antibody
    see all

Images

  • All lanes : Anti-PSF1 antibody [EPR13359] (ab181112) at 1/20000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling PSF1 with ab181112 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Western blot analysis of PSF1 in HepG2 cell lysate immunoprecipitated with ab181112 at 1/50 dilution (Lane 1). Lane 2: Negative control.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab181112 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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