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Rabbit monoclonal [EPR13359] to PSF1
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PSF1 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q14691
Jurkat, HeLa and HepG2 cell lysates; HepG2 cells.
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
1/250 - 1/500.
The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS1 is essential for function.
Belongs to the GINS1/PSF1 family.
Information by UniProt
DNA replication complex GINS protein PSF1 antibody
GINS complex subunit 1 (Psf1 homolog) antibody
GINS complex subunit 1 antibody
Western blot - Anti-PSF1 [EPR13359] antibody (ab181112)
All lanes : Anti-PSF1 antibody [EPR13359] (ab181112) at 1/20000 dilution Lane 1 : Jurkat cell lysate Lane 2 : HeLa cell lysate Lane 3 : HepG2 cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution Predicted band size : 23 kDa Observed band size : 23 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PSF1 [EPR13359] antibody (ab181112)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling PSF1 with ab181112 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Immunoprecipitation - Anti-PSF1 [EPR13359] antibody (ab181112)
Western blot analysis of PSF1 in HepG2 cell lysate immunoprecipitated with ab181112 at 1/50 dilution (Lane 1). Lane 2: Negative control.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"