Overview

  • Product name
  • Description
    Rabbit polyclonal to PSG9
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: VYSNASLLIQ NVTRKDAGTY TLHIIKRGDE TREEIRHFTF TLYLETPKPY , corresponding to amino acids 101-150 of Human PSG9

  • Positive control
    • HepG2 cell lysate,

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Sequence similarities
    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage
    PSBG are produced in high quantity during pregnancy.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • pregnancy specific beta 1 glycoprotein (PSG) antibody
    • pregnancy specific beta 1 glycoprotein 11 antibody
    • pregnancy specific beta 1 glycoprotein 9 antibody
    • pregnancy specific beta 1 glycoprotein B antibody
    • Pregnancy-specific beta-1 glycoprotein B antibody
    • Pregnancy-specific beta-1-glycoprotein 11 antibody
    • Pregnancy-specific beta-1-glycoprotein 9 antibody
    • Pregnancy-specific glycoprotein 11 antibody
    • Pregnancy-specific glycoprotein 7 antibody
    • Pregnancy-specific glycoprotein 9 antibody
    • PS beta B antibody
    • PS-beta-B antibody
    • PS-beta-G-11 antibody
    • PS-beta-G-9 antibody
    • PS34 antibody
    • PSBG 9 antibody
    • PSBG-11 antibody
    • PSBG-9 antibody
    • PSG 9 antibody
    • PSG11 antibody
    • PSG7 antibody
    • PSG9 antibody
    • PSG9_HUMAN antibody
    • PSGII antibody
    see all

Images

  • Anti-PSG9 antibody (ab64425) at 0.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ICC/IF image of ab64425 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64425, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab64425 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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