Overview

  • Product nameAnti-PSKH1 antibody
    See all PSKH1 primary antibodies
  • Description
    Rabbit polyclonal to PSKH1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 1-188 of Human PSKH1 (AAH62616).

  • Positive control
    • WB: MOLT4 whole cell lysate; 293T, A431, H1299, HeLa S3, HepG2 and Raji cell lines ICC/IF: Hela cell

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionMay be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.
  • Tissue specificityExpressed in all tissues and cell lines tested with the highest level of abundance in testis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on serine residues.
    Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur.
    Palmitoylated.
  • Cellular localizationGolgi apparatus. Cytoplasm > cytoskeleton > centrosome. Nucleus speckle. Endoplasmic reticulum membrane. Cell membrane. Cytoplasm. Localized in the brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPSH1_HUMAN antibody
    • Protein serine kinase H1 antibody
    • PSK H1 antibody
    • PSK-H1 antibody
    • PSKH1 antibody
    • Serine/threonine protein kinase H1 antibody
    • Serine/threonine-protein kinase H1 antibody
    see all

Anti-PSKH1 antibody images

  • Anti-PSKH1 antibody (ab96602) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 48 kDa
  • ab96602, at 1/200 dilution, staining PSKH1 in paraformaldehyde fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

References for Anti-PSKH1 antibody (ab96602)

ab96602 has not yet been referenced specifically in any publications.

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