Overview

  • Product nameAnti-PSMA antibody [EP3253]
    See all PSMA primary antibodies
  • Description
    Rabbit monoclonal [EP3253] to PSMA
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of human PSMA

  • Positive control
    • LnCaP cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 84 kDa.
IP 1/10.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionHas both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
      Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
    • Tissue specificityHighly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
    • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
    • DomainThe NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
    • Post-translational
      modifications
      The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
      The N-terminus is blocked.
    • Cellular localizationCytoplasm and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell growth inhibiting protein 27 antibody
      • Cell growth-inhibiting gene 27 protein antibody
      • FGCP antibody
      • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
      • Folate hydrolase 1 antibody
      • Folate hydrolase antibody
      • Folate hydrolase prostate specific membrane antigen 1 antibody
      • FOLH 1 antibody
      • FOLH antibody
      • Folh1 antibody
      • FOLH1_HUMAN antibody
      • Folylpoly gamma glutamate carboxypeptidase antibody
      • Folylpoly-gamma-glutamate carboxypeptidase antibody
      • GCP 2 antibody
      • GCP II antibody
      • GCP2 antibody
      • GCPII antibody
      • GIG27 antibody
      • Glutamate carboxylase II antibody
      • Glutamate carboxypeptidase 2 antibody
      • Glutamate carboxypeptidase II antibody
      • Membrane glutamate carboxypeptidase antibody
      • mGCP antibody
      • N acetylated alpha linked acidic dipeptidase 1 antibody
      • N-acetylated-alpha-linked acidic dipeptidase I antibody
      • NAALAD 1 antibody
      • NAALAD1 antibody
      • NAALAdase antibody
      • NAALADase I antibody
      • Prostate specific membrane antigen antibody
      • Prostate specific membrane antigen variant F antibody
      • Prostate-specific membrane antigen antibody
      • PSM antibody
      • PSMA antibody
      • Pteroylpoly gamma glutamate carboxypeptidase antibody
      • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
      see all

    Anti-PSMA antibody [EP3253] images

    • Anti-PSMA antibody [EP3253] (ab76104) at 1/5000 dilution + LnCaP cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 84 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    References for Anti-PSMA antibody [EP3253] (ab76104)

    ab76104 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76104.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"