Anti-PSMA antibody [GCP-05] (ab66912)

Overview

  • Product nameAnti-PSMA antibody [GCP-05]
    See all PSMA primary antibodies
  • Description
    Mouse monoclonal [GCP-05] to PSMA
  • Tested applicationsSuitable for: IP, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 44-750 of Human PSMA

  • EpitopeThis antibody recognizes the extracellular domain of PSMA.
  • Positive control
    • LNCaP cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab66912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 - 5 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionHas both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
    Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
  • Tissue specificityHighly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
  • DomainThe NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
  • Post-translational
    modifications
    The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
    The N-terminus is blocked.
  • Cellular localizationCytoplasm and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell growth inhibiting protein 27 antibody
    • Cell growth-inhibiting gene 27 protein antibody
    • FGCP antibody
    • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
    • Folate hydrolase 1 antibody
    • Folate hydrolase antibody
    • Folate hydrolase prostate specific membrane antigen 1 antibody
    • FOLH 1 antibody
    • FOLH antibody
    • Folh1 antibody
    • FOLH1_HUMAN antibody
    • Folylpoly gamma glutamate carboxypeptidase antibody
    • Folylpoly-gamma-glutamate carboxypeptidase antibody
    • GCP 2 antibody
    • GCP II antibody
    • GCP2 antibody
    • GCPII antibody
    • GIG27 antibody
    • Glutamate carboxylase II antibody
    • Glutamate carboxypeptidase 2 antibody
    • Glutamate carboxypeptidase II antibody
    • Membrane glutamate carboxypeptidase antibody
    • mGCP antibody
    • N acetylated alpha linked acidic dipeptidase 1 antibody
    • N-acetylated-alpha-linked acidic dipeptidase I antibody
    • NAALAD 1 antibody
    • NAALAD1 antibody
    • NAALAdase antibody
    • NAALADase I antibody
    • Prostate specific membrane antigen antibody
    • Prostate specific membrane antigen variant F antibody
    • Prostate-specific membrane antigen antibody
    • PSM antibody
    • PSMA antibody
    • Pteroylpoly gamma glutamate carboxypeptidase antibody
    • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
    see all

Anti-PSMA antibody [GCP-05] images

  • This image shows LNCaP cell line stained by ab66911 and ab66912. Green column - antibodies at 1µg/ml; Blue column - antibodies at 5µg/ml; Yellow column - negative cell line.

References for Anti-PSMA antibody [GCP-05] (ab66912)

ab66912 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Unfortunately ab66912 is not tissue specific. I did however search our catalog and have found 2 products which may suit your needs. When searching for prostate cancer specific PSMA antibodies I found that the iso...

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Thank you for contacting us.

We do not have anti PSMA antibody that would detect both the native as well as reduced form of protein. We however have more than 20 products that are used under reducing conditions and gave best results. Taking ...

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