Anti-PSMA antibody [GCP-05] (Phycoerythrin) (ab77228)


  • Product nameAnti-PSMA antibody [GCP-05] (Phycoerythrin)
    See all PSMA primary antibodies
  • Description
    Mouse monoclonal [GCP-05] to PSMA (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab77228 recognizes extracellular domain of PSMA, expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 44-750 of Human PSMA

  • General notes

    The antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.



Our Abpromise guarantee covers the use of ab77228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionHas both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
    Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
  • Tissue specificityHighly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
  • DomainThe NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
  • Post-translational
    The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
    The N-terminus is blocked.
  • Cellular localizationCytoplasm and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell growth inhibiting protein 27 antibody
    • Cell growth-inhibiting gene 27 protein antibody
    • FGCP antibody
    • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
    • Folate hydrolase 1 antibody
    • Folate hydrolase antibody
    • Folate hydrolase prostate specific membrane antigen 1 antibody
    • FOLH 1 antibody
    • FOLH antibody
    • Folh1 antibody
    • FOLH1_HUMAN antibody
    • Folylpoly gamma glutamate carboxypeptidase antibody
    • Folylpoly-gamma-glutamate carboxypeptidase antibody
    • GCP 2 antibody
    • GCP II antibody
    • GCP2 antibody
    • GCPII antibody
    • GIG27 antibody
    • Glutamate carboxylase II antibody
    • Glutamate carboxypeptidase 2 antibody
    • Glutamate carboxypeptidase II antibody
    • Membrane glutamate carboxypeptidase antibody
    • mGCP antibody
    • N acetylated alpha linked acidic dipeptidase 1 antibody
    • N-acetylated-alpha-linked acidic dipeptidase I antibody
    • NAALAD 1 antibody
    • NAALAD1 antibody
    • NAALAdase antibody
    • NAALADase I antibody
    • Prostate specific membrane antigen antibody
    • Prostate specific membrane antigen variant F antibody
    • Prostate-specific membrane antigen antibody
    • PSM antibody
    • PSMA antibody
    • Pteroylpoly gamma glutamate carboxypeptidase antibody
    • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
    see all

References for Anti-PSMA antibody [GCP-05] (Phycoerythrin) (ab77228)

This product has been referenced in:
  • Sácha P  et al. Expression of glutamate carboxypeptidase II in human brain. Neuroscience 144:1361-72 (2007). Read more (PubMed: 17150306) »
  • Barinka C  et al. Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding. Eur J Biochem 271:2782-90 (2004). Read more (PubMed: 15206943) »

See all 4 Publications for this product

Product Wall

I can confirm that there is only one Phycoerythrin molecule conjugated to one antibody molecule in this product (ab77228).

For western blotting with GCP-05 with LnCaP cells, one just needs a standard whole cell extract. There is no LnCaP-focused procedure that I am aware of.

Regarding references, all publications we are aware of that have used this anti...

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Thank you for contacting us. The testing by the laboratory was done using LNCaP cells, so these would be a good positive control as well as the cell types used in the references.

I hope this information is helpful to you. Please do not hesit...

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Thank you for contacting us.

After review of our catalog and discussion with the lab, I am sorry to inform you that our current anti-PSMA products appear to either be conformationally independent or not tested for conformational bias. Whil...

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Thank you for contacting us.

We do not have anti PSMA antibody that would detect both the native as well as reduced form of protein. We however have more than 20 products that are used under reducing conditions and gave best results. Taking ...

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Thank you for contacting us and for reporting the problem you experienced with the antibodies ab66911 and ab77228. I am sorry that the product you received did not perform as stated on the datasheet. We will use the data you provided to investigate ...

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Thank you for your inquiry, and for your patience while we looked into this. The fluorochrome ratio for this antibody is 1:1. I hope this helps. Please do not hesitate to contact us if you need any additional assistanc