Recombinant
RabMAb

Anti-PSMA4 [EPR5831(2)] antibody (ab191403)

Overview

  • Product name
    Anti-PSMA4 [EPR5831(2)] antibody
    See all PSMA4 primary antibodies
  • Description
    Rabbit monoclonal [EPR5831(2)] to PSMA4
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PSMA4 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P25789

  • Positive control
    • Jurkat, K562, HeLa, Jar, rat C6, rat PC12 and mouse NIH 3T3 cell lysates. Human papillary adenocarcinoma of thyroid and mouse liver tissues. HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR5831(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/200.

Target

  • Function
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
  • Sequence similarities
    Belongs to the peptidase T1A family.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm, P-body. Colocalizes with TRIM5 in the cytoplasmic bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • HC9 antibody
    • HsT17706 antibody
    • Macropain subunit C9 antibody
    • MGC111191 antibody
    • MGC12467 antibody
    • MGC24813 antibody
    • Multicatalytic endopeptidase complex subunit C9 antibody
    • Proteasome (prosome macropain) subunit alpha type 4 antibody
    • Proteasome alpha 4 subunit antibody
    • Proteasome component C9 antibody
    • Proteasome subunit alpha type 4 antibody
    • Proteasome subunit alpha type-4 antibody
    • Proteasome subunit HC9 antibody
    • Proteasome subunit L antibody
    • PSA4_HUMAN antibody
    • PSC9 antibody
    • PSMA 4 antibody
    • psmA4 antibody
    see all

Images

  • All lanes : Anti-PSMA4 [EPR5831(2)] antibody (ab191403) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • All lanes : Anti-PSMA4 [EPR5831(2)] antibody (ab191403) at 1/1000 dilution

    Lane 1 : rat C6 cell lysate
    Lane 2 : mouse Raw 264.7 cell lysate
    Lane 3 : rat PC12 cell lysate
    Lane 4 : mouse NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • Immunohistochemical analysis of paraffin-embedded, Human papillary adenocarcinoma of thyroid tissue labeling PSMA4 with ab191403 at a 1/2000 dilution (1μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

  • Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling PSMA4 with ab191403 at a 1/2000 dilution (1μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling PSMA4 with ab191403 at a 1/500 dilution ( 4 ug/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PSMA4 at a 1/500 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

  • Western blot analysis on immunoprecipitation from 1) Jurkat cell lysate and 2) PBS, labeling PSMA4 using ab191403 at 1/200 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

References

ab191403 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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