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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
Overview
Product name
Anti-PSMB5 antibody
See all PSMB5 products (5) ...
Description
Rabbit polyclonal to PSMB5
Specificity
Detects proteasome PSMB5.
Tested applications
WB, IHC-Fr, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Cow, Human
Immunogen
Synthetic Peptide: CW(243)IRVSSDNVADLHDKYS(259). Immunizin g peptide corresponds to amino acid residues 243-259 from mouse proteasome PSMB5. This sequence is 94% conserved in human and rat.
C-WIRVSSDN VADLHDKYS
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSMB5 antibody (ab3330)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab3330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 22 kDa.(PubMed: 17008387)
IHC-Fr: 1/500(PubMed: 17008387)
IHC-P: Use a concentration of 4 µg/ml
ICC/IF: Use at an assay dependent dilution. (PubMed: 21059646)
Target
Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage throught the Nrf2-ARE pathway.
Sequence similarities
Belongs to the peptidase T1B family.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP28074
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp459C139 antibody
- EC 3.4.25.1 antibody
- LMPX antibody
see all
Database links
- Entrez Gene: 534640 Cow
- Entrez Gene: 5693 Human
- Entrez Gene: 19173 Mouse
- Omim: 600306 Human
- SwissProt: Q32KL2 Cow
- SwissProt: P28074 Human
- SwissProt: O55234 Mouse
- Unigene: 422990 Human
see all
Anti-PSMB5 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSMB5 antibody (ab3330)
ab3330 (4µg/ml) staining PSMB5 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartment of the intestinal glands cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-PSMB5 antibody (ab3330)
This product has been referenced in:
- Shen Xet al. Processing of optineurin in neuronal cells. J Biol Chem 286:3618-29 (2011). WB, ICC/IF; Mouse.Read more (PubMed: 21059646) »
- Pérez-Galán Pet al. Bortezomib resistance in mantle cell lymphoma is associated with plasmacytic differentiation. Blood 117:542-52 (2011). WB; Human.Read more (PubMed: 20956803) »
See all 3 publications for this product
Publishing research using ab3330? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"