Overview

  • Product nameAnti-PSMB5 antibody
    See all PSMB5 primary antibodies
  • Description
    Rabbit polyclonal to PSMB5
  • SpecificityDetects proteasome PSMB5.
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC-P, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse PSMB5 aa 243-259.
    Sequence:

    WIRVSSDNVADLHDKYS


    Database link: P28074
    (Peptide available as ab4946)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 22 kDa.Can be blocked with PSMB5 peptide (ab4946).
IHC-Fr 1/500. PubMed: 17008387
IHC-P Use a concentration of 4 µg/ml.
ICC 1/10 - 1/200.
ICC/IF 1/10 - 1/200.

Target

  • FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage throught the Nrf2-ARE pathway.
  • Sequence similaritiesBelongs to the peptidase T1B family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp459C139 antibody
    • EC 3.4.25.1 antibody
    • LMPX antibody
    • Macropain epsilon chain antibody
    • MB1 antibody
    • MGC104214 antibody
    • MGC118075 antibody
    • MGC134464 antibody
    • Multicatalytic endopeptidase complex epsilon chain antibody
    • Proteasome (prosome, macropain) subunit, beta type, 5 antibody
    • Proteasome beta 5 subunit antibody
    • Proteasome catalytic subunit 3 antibody
    • Proteasome chain 6 antibody
    • Proteasome epsilon chain antibody
    • Proteasome subunit beta type-5 antibody
    • Proteasome subunit MB1 antibody
    • Proteasome subunit X antibody
    • Proteasome subunit, beta type, 5 antibody
    • Proteasome subunit, beta-5 antibody
    • PSB5_HUMAN antibody
    • PSMB5 antibody
    • PSX large multifunctional protease X antibody
    • X antibody
    see all

Anti-PSMB5 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of PSMB5 (green) showing staining in the cytoplasm and nucleus of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescence analysis of PSMB5 (green) showing staining in the cytoplasm and nucleus of BAEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescence analysis of PSMB5 (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • All lanes : Anti-PSMB5 antibody (ab3330) at 1/1000 dilution

    Lane 1 : NCI-H1299 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Mouse liver cell lysate

    Lysates/proteins at 25 µg per lane.


    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
  • ab3330 (4µg/ml) staining PSMB5 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartment of the intestinal glands cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunofluorescence analysis of RGC5 cells, staining PSMB5 with ab3330 at 1/100 dilution.

     

References for Anti-PSMB5 antibody (ab3330)

This product has been referenced in:

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7 breast cancer cells)
Loading amount 20 µg
Specification MCF7 breast cancer cells
Treatment Control or PSMB5 siRNA transfected
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Purified protein (Liver and brain)
Loading amount 2 µg
Specification Liver and brain
Gel Running Conditions Reduced Denaturing (12,5% SDS gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Aug 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"