Overview

  • Product nameAnti-PSMC3 antibody
    See all PSMC3 primary antibodies
  • Description
    Rabbit polyclonal to PSMC3
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 71-120 (KIKENSEKIK VNKTLPYLVS NVIELLDVDP NDQEEDGANI DLDSQRKGKC) of Human PSMC3 (NP_002795).

  • Positive control
    • Placenta lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 31, 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.
  • Sequence similaritiesBelongs to the AAA ATPase family.
  • Post-translational
    modifications
    Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S protease regulatory subunit 6A antibody
    • 26S proteasome AAA-ATPase subunit RPT5 antibody
    • Human immunodeficiency virus tat transactivator binding protein 1 antibody
    • MGC8487 antibody
    • Proteasome (prosome macropain) 26S subunit ATPase 3 antibody
    • Proteasome 26S ATPase subunit 3 antibody
    • Proteasome 26S subunit ATPase 3 antibody
    • Proteasome subunit P50 antibody
    • PRS6A_HUMAN antibody
    • PSMC 3 antibody
    • PSMC3 antibody
    • Tat binding protein 1 antibody
    • TAT-binding protein 1 antibody
    • TBP-1 antibody
    • TBP1 antibody
    see all

Anti-PSMC3 antibody images

  • Anti-PSMC3 antibody (ab83348) at 1 µg/ml + placenta lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 31,49 kDa (why is the actual band size different from the predicted?)

References for Anti-PSMC3 antibody (ab83348)

ab83348 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"