Overview

  • Product name
  • Description
    Rabbit polyclonal to PSMC3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 51-331 of Human PSMC3 (NP_002795).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Raji cell lysates; Molt-4 whole cell lysate (WB); A549 cells (IF); Cal27 xenograft (IHC)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.
  • Sequence similarities
    Belongs to the AAA ATPase family.
  • Post-translational
    modifications
    Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S protease regulatory subunit 6A antibody
    • 26S proteasome AAA-ATPase subunit RPT5 antibody
    • Human immunodeficiency virus tat transactivator binding protein 1 antibody
    • MGC8487 antibody
    • Proteasome (prosome macropain) 26S subunit ATPase 3 antibody
    • Proteasome 26S ATPase subunit 3 antibody
    • Proteasome 26S subunit ATPase 3 antibody
    • Proteasome subunit P50 antibody
    • PRS6A_HUMAN antibody
    • PSMC 3 antibody
    • PSMC3 antibody
    • Tat binding protein 1 antibody
    • TAT-binding protein 1 antibody
    • TBP-1 antibody
    • TBP1 antibody
    see all

Images

  • Anti-PSMC3 antibody (ab96558) at 1/5000 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size : 49 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab96558 at 1/200 dilution. Lower image merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab96558 at 1/500 dilution.

References

ab96558 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab96558.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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