Recombinant
RabMAb

Anti-PSMC3 antibody [EPR12152] - N-terminal (ab171969)

Overview

  • Product name
    Anti-PSMC3 antibody [EPR12152] - N-terminal
    See all PSMC3 primary antibodies
  • Description
    Rabbit monoclonal [EPR12152] to PSMC3 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PSMC3 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: P17980

  • Positive control
    • Human ovarian carcinoma and tonsil tissues. 293 cells. Human placenta, 293T, HeLa and HepG2 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12152
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 49 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.

Target

  • Function
    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation.
  • Sequence similarities
    Belongs to the AAA ATPase family.
  • Post-translational
    modifications
    Sumoylated by UBE2I in response to MEKK1-mediated stimuli.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S protease regulatory subunit 6A antibody
    • 26S proteasome AAA-ATPase subunit RPT5 antibody
    • Human immunodeficiency virus tat transactivator binding protein 1 antibody
    • MGC8487 antibody
    • Proteasome (prosome macropain) 26S subunit ATPase 3 antibody
    • Proteasome 26S ATPase subunit 3 antibody
    • Proteasome 26S subunit ATPase 3 antibody
    • Proteasome subunit P50 antibody
    • PRS6A_HUMAN antibody
    • PSMC 3 antibody
    • PSMC3 antibody
    • Tat binding protein 1 antibody
    • TAT-binding protein 1 antibody
    • TBP-1 antibody
    • TBP1 antibody
    see all

Images

  • Western blot analysis on immunoprecipitation pellet from (Lane 1) HeLa cell lysate or (Lane 2) 1X PBS (negative control) using ab171969 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • All lanes : Anti-PSMC3 antibody [EPR12152] - N-terminal (ab171969) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 49 kDa

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling PSMC3 using ab171969 at a 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue, labeling PSMC3 using ab171969 at a 1/50 dilution.

  • Immunofluorescence analysis of 293 cells labeling PSMC3 using ab171969 at a 1/250 dilution.

  • Flow cytometric analysis of permeabilized 293 cells, labeling PSMC3 using ab171969 at a 1/10 dilution (red) or a rabbit IgG negative control (green).

References

ab171969 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171969.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up