Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node tissue labelling PSMC5 with ab70832 at 1/200 (1µg/ml). Detection: DAB.
Western blot - PSMC5 antibody (ab70832)
All lanes : Anti-PSMC5 antibody (ab70832) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg
developed using the ECL technique
Predicted band size : 46 kDa Observed band size : 46 kDa
Exposure time : 30 seconds
Immunoprecipitation - PSMC5 antibody (ab70832)
Detection of Human PSMC5 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells, using ab70832 at 3 µg/mg lysate (Lane 1). Lane 2 represents control IgG IP. Subsequent WB detection was performed using 1 µg/ml ab70832 and chemiluminescence with an exposure time of 30 seconds.
References for Anti-PSMC5 antibody (ab70832)
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