Overview

  • Product name
    Anti-PSMC6 antibody [p42-23]
    See all PSMC6 primary antibodies
  • Description
    Mouse monoclonal [p42-23] to PSMC6
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • HeLa S3 cytosolic preparation and human placental proteasome preparation.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01M Sodium Azide
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Partially purified immunoglobulin preparation.
  • Clonality
    Monoclonal
  • Clone number
    p42-23
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/1000. PubMed: 18986984
WB Use at an assay dependent concentration. Detects a band of approximately 44 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
    • Sequence similarities
      Belongs to the AAA ATPase family.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 26S protease regulatory subunit 10B antibody
      • 26S protease regulatory subunit S10B antibody
      • 26S proteasome AAA-ATPase subunit RPT4 antibody
      • CADP44 antibody
      • P44 antibody
      • Proteasome 26S subunit ATPase 6 antibody
      • Proteasome subunit p42 antibody
      • PRS10_HUMAN antibody
      • PSMC6 antibody
      • Rpt4 antibody
      • SUG2 antibody
      see all

    Anti-PSMC6 antibody [p42-23] images

    • Lane 1 : Anti-PSMC6 antibody [p42-23] (ab22639) at 1/5000 dilution (Identification of 44KDa Rpt4 in HeLa S3 cytosolic preparation)
      Lane 2 : Anti-PSMC6 antibody [p42-23] (ab22639) at 1/5000 dilution (Identificaition of purified protein)
      Lane 3 : Anti-PSMC6 antibody [p42-23] (ab22639) at 1/5000 dilution (Identification of proteasome in human placenta )

      Lane 1 : As above
      Lane 2 : As above
      Lane 3 : As above

      Developed using the ECL technique

      Observed band size : 44 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute
    • ab22639 staining PSMC6 in mock transfected rat primary neurons by Immunocytochemistry/ Immunofluorescence. Primary cultures of rat hippocampal neurons were prepared from E18 rats. Cells were fixed in 4% paraformaldehyde and incubated with primary antibody at 1/1000 dilution. The image show red staining with ab22639, nucleus was stained blue with Hoechst H33258 and right image show merged images. The green color seen in right image is the GFP staining in similar experiment.

    • IHC image of ab22639 staining in human normal pancrease formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22639, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Overlay histogram showing HeLa cells stained with ab22639 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22639, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-PSMC6 antibody [p42-23] (ab22639)

    This product has been referenced in:
    • Rousseau E  et al. Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones. J Biol Chem 284:1917-29 (2009). WB, ICC/IF ; Human . Read more (PubMed: 18986984) »
    • Hendil KB  et al. Simultaneous binding of PA28 and PA700 activators to 20 S proteasomes. Biochem J 332 ( Pt 3):749-54 (1998). Read more (PubMed: 9620878) »

    See all 2 Publications for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Caenorhabditis elegans Cell (2 cell embryos)
    Permeabilization
    Yes - PBS + 0.05% Tween
    Specification
    2 cell embryos
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Fixative
    Methanol/Acetone
    Username

    Abcam user community

    Verified customer

    Submitted May 20 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (liver)
    Loading amount
    30 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (4-20% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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