Anti-PSMC6 antibody [p42-23] (ab22639)
Key features and details
- Mouse monoclonal [p42-23] to PSMC6
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-PSMC6 antibody [p42-23]
See all PSMC6 primary antibodies -
Description
Mouse monoclonal [p42-23] to PSMC6 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (Human).
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Positive control
- HeLa S3 cytosolic preparation and human placental proteasome preparation.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Partially purified immunoglobulin preparation. -
Clonality
Monoclonal -
Clone number
p42-23 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 44 kDa.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Detects a band of approximately 44 kDa. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. -
Sequence similarities
Belongs to the AAA ATPase family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5706 Human
- Omim: 602708 Human
- SwissProt: P62333 Human
- Unigene: 156171 Human
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Alternative names
- 26S protease regulatory subunit 10B antibody
- 26S protease regulatory subunit S10B antibody
- 26S proteasome AAA-ATPase subunit RPT4 antibody
see all
Images
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All lanes : Anti-PSMC6 antibody [p42-23] (ab22639) at 1/5000 dilution
Lane 1 : Rpt4 in HeLa S3 cytosolic preparation
Lane 2 : Purified protein
Lane 3 : Proteasome in human placenta
Developed using the ECL technique.
Observed band size: 44 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
IHC image of ab22639 staining in human normal pancrease formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22639, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Overlay histogram showing HeLa cells stained with ab22639 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22639, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab22639 has been referenced in 5 publications.
- Gizak A et al. A novel remitting leukodystrophy associated with a variant in FBP2. Brain Commun 3:fcab036 (2021). PubMed: 33977262
- Tao P et al. Deubiquitination of proteasome subunits by OTULIN regulates type I IFN production. Sci Adv 7:eabi6794 (2021). PubMed: 34797715
- Ahmed S et al. Small-scale isolation of synaptic vesicles from mammalian brain. Nat Protoc 8:998-1009 (2013). PubMed: 23619891
- Rousseau E et al. Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones. J Biol Chem 284:1917-29 (2009). WB, ICC/IF ; Human . PubMed: 18986984
- Hendil KB et al. Simultaneous binding of PA28 and PA700 activators to 20 S proteasomes. Biochem J 332 ( Pt 3):749-54 (1998). PubMed: 9620878