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Our Abpromise guarantee covers the use of ab2941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).
By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract.
|IHC-Fr||Use a concentration of 15 µg/ml.|
|IHC-P||Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
Western blot of proteasome Rpn2 from HeLa cell extract using ab2941.