Anti-PSMD11 antibody [AT1F4] (ab89599)

Overview

  • Product nameAnti-PSMD11 antibody [AT1F4]
    See all PSMD11 primary antibodies
  • Description
    Mouse monoclonal [AT1F4] to PSMD11
  • Tested applicationsSuitable for: ICC/IF, WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length Human PSMD11 purified from E. coli, amino acids 1-422 (NP_002806)

  • Positive control
    • Mouse kidney cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberAT1F4
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/250 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Indirect ELISA Use at an assay dependent dilution.

Target

  • FunctionComponent of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
  • Tissue specificityHighly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
  • Sequence similaritiesBelongs to the proteasome subunit S9 family.
    Contains 1 PCI domain.
  • Post-translational
    modifications
    Phosphorylated by AMPK.
  • Cellular localizationNucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S proteasome non-ATPase regulatory subunit 11 antibody
    • 26S proteasome regulatory subunit 9 antibody
    • 26S proteasome regulatory subunit p44.5 antibody
    • 26S proteasome regulatory subunit RPN6 antibody
    • 26S proteasome regulatory subunit S9 antibody
    • MGC3844 antibody
    • p44.5 antibody
    • protease 26S, subunit, 9 antibody
    • proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 antibody
    • proteasome 26S subunit, non-ATPase, 11 antibody
    • PSD11_HUMAN antibody
    • PSMD 11 antibody
    • PSMD11 antibody
    • Rpn6 antibody
    • S9 antibody
    see all

Anti-PSMD11 antibody [AT1F4] images

  • Anti-PSMD11 antibody [AT1F4] (ab89599) at 1/250 dilution + mouse kidney extracts at 60 µg

    Secondary
    goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

References for Anti-PSMD11 antibody [AT1F4] (ab89599)

ab89599 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (HEK293)
Specification HEK293
Permeabilization Yes - triton X-100
Fixative Paraformaldehyde
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Submitted Jul 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"