The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/250 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Use at an assay dependent dilution.
FunctionComponent of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Tissue specificityHighly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
Sequence similaritiesBelongs to the proteasome subunit S9 family. Contains 1 PCI domain.
Post-translational modificationsPhosphorylated by AMPK.