Overview

  • Product name
  • Description
    Rabbit polyclonal to PSMD14
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human PSMD14 aa 216-310. The exact sequence is proprietary. (With tag).
    Database link: O00487

  • Positive control
    • 293T, SKOV3, 231 and mouse NIH 3T3 cell lysates; Human liver cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab182762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 35 kDa.

Target

  • Function
    Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
  • Tissue specificity
    Widely expressed. Highest levels in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the peptidase M67A family. PSMD14 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S proteasome non-ATPase regulatory subunit 14 antibody
    • 26S proteasome regulatory subunit rpn11 antibody
    • 26S proteasome-associated PAD1 homolog 1 antibody
    • 26S proteasome-associated PAD1 homolog antibody
    • PAD1 antibody
    • PAD1, yeast, homolog of antibody
    • POH1 antibody
    • Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody
    • Proteasome 26S subunit non ATPase 14 antibody
    • PSDE_HUMAN antibody
    • Psmd14 antibody
    • RPN11 antibody
    • Testis tissue sperm binding protein Li 69n antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling PSMD14 with ab182762 at 1/20 dilution (left panel). Right panel is treated with the immunogen.

  • All lanes : Anti-PSMD14 antibody (ab182762) at 1/200 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : SKOV3 cell lysate
    Lane 3 : NIH 3T3 cell lysate
    Lane 4 : 231 cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 35 kDa


    Exposure time : 1 minute

    Gel: 10% SDS-PAGE

References

ab182762 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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