Recombinant
RabMAb

Anti-PSMD14 antibody [EPR4257] (ab109123)

Overview

  • Product name
    Anti-PSMD14 antibody [EPR4257]
    See all PSMD14 primary antibodies
  • Description
    Rabbit monoclonal [EPR4257] to PSMD14
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PSMD14.

  • Positive control
    • HepG2, A431, HeLa, Namalwa, K562 and MCF7 cell lysates; Human colon tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).
ICC/IF 1/500 - 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
    • Tissue specificity
      Widely expressed. Highest levels in heart and skeletal muscle.
    • Sequence similarities
      Belongs to the peptidase M67A family. PSMD14 subfamily.
      Contains 1 MPN (JAB/Mov34) domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 26S proteasome non-ATPase regulatory subunit 14 antibody
      • 26S proteasome regulatory subunit rpn11 antibody
      • 26S proteasome-associated PAD1 homolog 1 antibody
      • 26S proteasome-associated PAD1 homolog antibody
      • PAD1 antibody
      • PAD1, yeast, homolog of antibody
      • POH1 antibody
      • Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody
      • Proteasome 26S subunit non ATPase 14 antibody
      • PSDE_HUMAN antibody
      • Psmd14 antibody
      • RPN11 antibody
      • Testis tissue sperm binding protein Li 69n antibody
      see all

    Images

    • All lanes : Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : Namalwa cell lysates
      Lane 5 : K562 cell lysates
      Lane 6 : MCF7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
    • ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
    • ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.

    References

    ab109123 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Purified protein (protein purified from yeast or recombinantly expre)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycine Biorad Stain free)
    Loading amount
    0.4 µg
    Specification
    protein purified from yeast or recombinantly expre
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
    Username

    Ellen Goodall

    Verified customer

    Submitted Aug 01 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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