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Product name Anti-PSMD14 antibody [EPR4257] See all PSMD14 primary antibodies
Rabbit monoclonal [EPR4257] to PSMD14
Tested applications Suitable for:
WB, IHC-P, ICC/IF more details Unsuitable for:
Flow Cyt or IP
Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human PSMD14.
HepG2, A431, HeLa, Namalwa, K562 and MCF7 cell lysates; Human colon tissue; HeLa cells.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR4257
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).
1/500 - 1/1000.
Application notes Is unsuitable for Flow Cyt or IP.
Function Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
Tissue specificity Widely expressed. Highest levels in heart and skeletal muscle.
Sequence similarities Belongs to the peptidase M67A family. PSMD14 subfamily. Contains 1 MPN (JAB/Mov34) domain.
Information by UniProt
26S proteasome non-ATPase regulatory subunit 14 antibody
26S proteasome regulatory subunit rpn11 antibody
26S proteasome-associated PAD1 homolog 1 antibody
Anti-PSMD14 antibody [EPR4257] images
Western blot - PSMD14 antibody [EPR4257] (ab109123)
All lanes : Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution Lane 1 : HepG2 cell lysates Lane 2 : A431 cell lysates Lane 3 : HeLa cell lysates Lane 4 : Namalwa cell lysates Lane 5 : K562 cell lysates Lane 6 : MCF7 cell lysates Lysates/proteins at 10 µg per lane. Secondary HRP labelled Goat anti-Rabbit at 1/2000 dilution Predicted band size : 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSMD14 antibody [EPR4257] (ab109123)
ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - PSMD14 antibody [EPR4257] (ab109123)
ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.
References for Anti-PSMD14 antibody [EPR4257] (ab109123)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"