Recombinant
RabMAb

Anti-PSMD14 antibody [EPR4257] (ab109123)

Overview

  • Product name
    Anti-PSMD14 antibody [EPR4257]
    See all PSMD14 primary antibodies
  • Description
    Rabbit monoclonal [EPR4257] to PSMD14
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PSMD14.

  • Positive control
    • HepG2, A431, HeLa, Namalwa, K562 and MCF7 cell lysates; Human colon tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).
ICC/IF 1/500 - 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
    • Tissue specificity
      Widely expressed. Highest levels in heart and skeletal muscle.
    • Sequence similarities
      Belongs to the peptidase M67A family. PSMD14 subfamily.
      Contains 1 MPN (JAB/Mov34) domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 26S proteasome non-ATPase regulatory subunit 14 antibody
      • 26S proteasome regulatory subunit rpn11 antibody
      • 26S proteasome-associated PAD1 homolog 1 antibody
      • 26S proteasome-associated PAD1 homolog antibody
      • PAD1 antibody
      • PAD1, yeast, homolog of antibody
      • POH1 antibody
      • Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody
      • Proteasome 26S subunit non ATPase 14 antibody
      • PSDE_HUMAN antibody
      • Psmd14 antibody
      • RPN11 antibody
      • Testis tissue sperm binding protein Li 69n antibody
      see all

    Anti-PSMD14 antibody [EPR4257] images

    • All lanes : Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : Namalwa cell lysates
      Lane 5 : K562 cell lysates
      Lane 6 : MCF7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
    • ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
    • ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.

    References for Anti-PSMD14 antibody [EPR4257] (ab109123)

    ab109123 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Purified protein (protein purified from yeast or recombinantly expre)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycine Biorad Stain free)
    Loading amount
    0.4 µg
    Specification
    protein purified from yeast or recombinantly expre
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
    Username

    Ellen Goodall

    Verified customer

    Submitted Aug 01 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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