Overview

  • Product nameAnti-PSMD7 antibody
    See all PSMD7 primary antibodies
  • Description
    Rabbit polyclonal to PSMD7
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
    Predicted to work with: Cow, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human PSMD7 aa 55-66.
    Sequence:

    AVPFDEDDKDDS


    (Peptide available as ab41795)

  • Positive control
    • K562 whole cell lysate and other human, rat, mouse and hamster cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab11436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa.Can be blocked with Human PSMD7 peptide (ab41795).

Target

  • FunctionActs as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • Sequence similaritiesBelongs to the peptidase M67A family.
    Contains 1 MPN (JAB/Mov34) domain.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 26S proteasome non ATPase regulatory subunit 7 antibody
    • 26S proteasome non-ATPase regulatory subunit 7 antibody
    • 26S proteasome regulatory subunit RPN8 antibody
    • 26S proteasome regulatory subunit S12 antibody
    • integration site gene, mouse, homolog of antibody
    • Moloney leukemia virus 34 proviral antibody
    • Moloney leukemia virus 34 proviral integration antibody
    • MOV34 antibody
    • Mov34 homolog antibody
    • Mov34 protein homolog antibody
    • MOV34L antibody
    • P40 antibody
    • Proteasome (prosome, macropain) 26S subunit, non ATPase 7 antibody
    • Proteasome (prosome, macropain) 26S subunit, non ATPase, 7 (Mov34 homolog) antibody
    • Proteasome 26S S12 antibody
    • Proteasome 26S subunit, no-ATPase, 7 antibody
    • Proteasome subunit p40 antibody
    • PSD7_HUMAN antibody
    • PSMD 7 antibody
    • PSMD7 antibody
    • Rpn8 antibody
    • S12 antibody
    see all

Anti-PSMD7 antibody images

  • Western blot of PSDM7 in K562 cell lysate using ab11436.
    Lane 1 is ab11436
    Lane 2 is ab11436 pre-incubated with peptide
  • IHC image of ab11436 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11436, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab11436 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11436, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PSMD7 antibody (ab11436)

ab11436 has not yet been referenced specifically in any publications.

Product Wall

Depending on the sample we occasionally see a band at about 48 kDa. If so, it is pretty normal. If the band is much closer to 37 kDa, and there is good separation of the proteins of this size on the gel, then this has not been observed previously. I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"