Overview

  • Product nameAnti-PSMD7 antibodySee all PSMD7 primary antibodies ...
  • Description
    Mouse polyclonal to PSMD7
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Silk worm
  • Immunogen

    Fusion protein: VPFDEDDKDD SVWFLDHDYL ENMYGMFKKV NARERIVGWY HTGPKLHKND IAINELMKRY CPNSVLVIID VKPKDLGLPT EAYISVEEVH DDGTPTSKTF, corresponding to amino acids 56-155 of Human Proteasome 26S S12 (PSMD7).

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab43402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/1000. Predicted molecular weight: 37 kDa.

    This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
    • Sequence similaritiesBelongs to the peptidase M67A family.
      Contains 1 MPN (JAB/Mov34) domain.
    • Target information above from: UniProt accession P51665 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 26S proteasome non ATPase regulatory subunit 7 antibody
      • 26S proteasome non-ATPase regulatory subunit 7 antibody
      • 26S proteasome regulatory subunit rpn8 antibody
      • 26S proteasome regulatory subunit S12 antibody
      • integration site gene, mouse, homolog of antibody
      • Moloney leukemia virus 34 proviral antibody
      • Moloney leukemia virus 34 proviral integration antibody
      • MOV34 antibody
      • Mov34 homolog antibody
      • Mov34 protein homolog antibody
      • MOV34L antibody
      • P40 antibody
      • Proteasome (prosome, macropain) 26S subunit, non ATPase 7 antibody
      • Proteasome (prosome, macropain) 26S subunit, non ATPase, 7 (Mov34 homolog) antibody
      • Proteasome 26S S12 antibody
      • Proteasome 26S subunit, no-ATPase, 7 antibody
      • Proteasome subunit p40 antibody
      • PSD7_HUMAN antibody
      • PSMD 7 antibody
      • PSMD7 antibody
      • Rpn8 antibody
      • S12 antibody
      see all

    References for Anti-PSMD7 antibody (ab43402)

    ab43402 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Dec 04 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"