Overview

  • Product nameAnti-PSME1 antibody
    See all PSME1 primary antibodies
  • Description
    Rabbit polyclonal to PSME1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PSME1 aa 220-249 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q06323

  • Positive control
    • HL60 cell line lysates; Human hepatocarcinoma tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab104098 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use 5µg for 10 cells.
WB 1/100 - 1/500. Predicted molecular weight: 29 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionImplicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
  • Sequence similaritiesBelongs to the PA28 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 11S regulator complex alpha subunit antibody
    • 11S regulator complex subunit alpha antibody
    • 29 kD MCP activator subunit antibody
    • 29kD MCP activator subunit antibody
    • Activator of multicatalytic protease subunit 1 antibody
    • IFI5111 antibody
    • IGUP I-5111 antibody
    • Interferon gamma IEF SSP 5111 antibody
    • Interferon gamma inducible protein 5111 antibody
    • Interferon gamma up regulated I 5111 protein antibody
    • Interferon gamma up-regulated I-5111 protein antibody
    • MGC8628 antibody
    • PA28a antibody
    • PA28alpha antibody
    • Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) antibody
    • Proteasome activator 28 subunit alpha antibody
    • Proteasome activator complex subunit 1 antibody
    • Proteasome activator subunit 1 antibody
    • PSME1 antibody
    • PSME1_HUMAN antibody
    • REG-alpha antibody
    • REGalpha antibody
    see all

Anti-PSME1 antibody images

  • ICC/IF image of ab104098 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104098 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PSME1 antibody (ab104098) at 1/100 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 29 kDa
  • ab104098 at 1/50 dilution, staining PMSE1 in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-PSME1 antibody (ab104098)

ab104098 has not yet been referenced specifically in any publications.

Product Wall

Gracias por contactarnos.

El anticuerpo ab104098 se ha purificado mediante columna de proteína A, seguido de purificación por afinidad con el inmunógeno inmovilizado en la columna.

Espero que esta informaci&oacu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"