Recombinant
RabMAb

Anti-PSME2 antibody [EPR14931] (ab183727)

Overview

  • Product name
    Anti-PSME2 antibody [EPR14931]
    See all PSME2 primary antibodies
  • Description
    Rabbit monoclonal [EPR14931] to PSME2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PSME2 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UL46

  • Positive control
    • HeLa, PC3 and HepG2 cell lysates; Human kidney clear cell carcinoma and stomach tissues; HeLa and HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14931
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ICC/IF 1/500.
Flow Cyt 1/210.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100.

Target

  • Function
    Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
  • Sequence similarities
    Belongs to the PA28 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 11S regulator complex beta subunit antibody
    • 11S regulator complex subunit beta antibody
    • Activator of multicatalytic protease subunit 2 antibody
    • Cell migration inducing protein 22 antibody
    • MCP activator 31 kD subunit antibody
    • PA28 beta antibody
    • PA28b antibody
    • PA28beta antibody
    • Proteasome (prosome macropain) activator subunit 2 (PA28 beta) antibody
    • Proteasome (prosome macropain) activator subunit 2 antibody
    • Proteasome activator 28 beta antibody
    • Proteasome activator 28 subunit beta antibody
    • Proteasome activator complex subunit 2 antibody
    • Proteasome activator hPA28 subunit beta antibody
    • Proteasome activator subunit 2 antibody
    • PSME 2 antibody
    • PSME2 antibody
    • PSME2_HUMAN antibody
    • REG beta antibody
    • REG-beta antibody
    • REGbeta antibody
    see all

Images

  • All lanes : Anti-PSME2 antibody [EPR14931] (ab183727) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC3 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 27 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling PSME2 with ab183727 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PSME2 with ab183727 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSME2 with ab183727 at 1/500 dilution, followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSME2 with ab183727 at 1/500 dilution, followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSME2 with ab183727 at 1/210 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

ab183727 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183727.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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