Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation.
Belongs to the PA28 family.
The C-terminal sequences affect heptamer stability and proteasome affinity.
Phosphorylated by MAP3K3.
Nucleus. Cytoplasm. Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.
Immunoprecipitation - Anti-PSME3 antibody (ab157157)This image is courtesy of an anonymous Abreview
ab157157 Immunoprecipitating PSME3 in human HEK293 whole cell lysate. 250µg of cell lysate was incubated with the undiluted primary antibody and matrix (Protein G) for 18 hours at 4°C. For western blotting ab157157 (1/1000) was used to confirm successful immunoprecipation.
All lanes : Anti-PSME3 antibody (ab157157) at 1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Detection of PMSE3 by Western Blot of Immunprecipitate.
ab157157 at 1µg/ml labeling PMSE3 in 293T whole cell lysate immunoprecipitated using ab157157 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes.