Anti-PSME3 antibody (ab157157)
Key features and details
- Rabbit polyclonal to PSME3
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PSME3 antibody
See all PSME3 primary antibodies -
Description
Rabbit polyclonal to PSME3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Zebra finch, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human PSME3 aa 200-300.
Database link: P61289 -
Positive control
- IP: HEK293 whole cell lysate. WB: 293T, HeLa, Jurkat and mouse NIH 3T3 whole cell lysates. IHC-P: Mouse renal carcinoma and human lung carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab157157 was affinity purified using an epitope specific to PSME3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/2500. Predicted molecular weight: 29 kDa.
|
IP | (1) |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/1000.
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Notes |
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WB
1/500 - 1/2500. Predicted molecular weight: 29 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/1000. |
Target
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Function
Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. -
Sequence similarities
Belongs to the PA28 family. -
Domain
The C-terminal sequences affect heptamer stability and proteasome affinity. -
Post-translational
modificationsPhosphorylated by MAP3K3. -
Cellular localization
Nucleus. Cytoplasm. Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase. - Information by UniProt
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Database links
- Entrez Gene: 420017 Chicken
- Entrez Gene: 740582 Chimpanzee
- Entrez Gene: 100336105 Cow
- Entrez Gene: 101131213 Gorilla
- Entrez Gene: 100065788 Horse
- Entrez Gene: 10197 Human
- Entrez Gene: 19192 Mouse
- Entrez Gene: 100171504 Orangutan
see all -
Alternative names
- 11S regulator complex gamma subunit antibody
- 11S regulator complex subunit gamma antibody
- Activator of multicatalytic protease subunit 3 antibody
see all
Images
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ab157157 Immunoprecipitating PSME3 in human HEK293 whole cell lysate. 250µg of cell lysate was incubated with the undiluted primary antibody and matrix (Protein G) for 18 hours at 4°C. For western blotting ab157157 (1/1000) was used to confirm successful immunoprecipation.
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All lanes : Anti-PSME3 antibody (ab157157) at 1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 3 minutes -
Detection of PMSE3 by Western Blot of Immunprecipitate.
ab157157 at 1µg/ml labeling PMSE3 in 293T whole cell lysate immunoprecipitated using ab157157 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 minutes. -
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal carcinoma tissue sections using ab117809 at 1/1000 dilution.
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Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections using ab117809 at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab157157 has been referenced in 5 publications.
- Manzoor M et al. Isoquercitrin from Apocynum venetum L. produces an anti-obesity effect on obese mice by targeting C-1-tetrahydrofolate synthase, carbonyl reductase, and glutathione S-transferase P and modification of the AMPK/SREBP-1c/FAS/CD36 signaling pathway in mice in vivo. Food Funct 13:10923-10936 (2022). PubMed: 36205648
- Xie Y et al. The proteasome activator REG? accelerates cardiac hypertrophy by declining PP2Aca-SOD2 pathway. Cell Death Differ N/A:N/A (2020). PubMed: 32424140
- Fan J et al. CKIP-1 limits foam cell formation and inhibits atherosclerosis by promoting degradation of Oct-1 by REG?. Nat Commun 10:425 (2019). PubMed: 30683852
- Shu Y et al. MicroRNA-195-5p inhibitor prevents the development of osteoarthritis by targeting REG?. Mol Med Rep 19:4561-4568 (2019). PubMed: 30942447
- Jia Q et al. Fisetin, via CKIP-1/REG?, limits oxidized LDL-induced lipid accumulation and senescence in RAW264.7 macrophage-derived foam cells. Eur J Pharmacol 865:172748 (2019). PubMed: 31655030