Recombinant
RabMAb

Anti-PSMG1 antibody [EPR10223] (ab167396)

Overview

  • Product name
    Anti-PSMG1 antibody [EPR10223]
    See all PSMG1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10223] to PSMG1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acid residues in Human PSMG1 (UniProt: O95456).

  • Positive control
    • U-87 MG, Jurkat, 293T and HepG2 cell lysates; Human brain and Human testis tissues; HepG2 cells;
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab167396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance
      PSMG1 is a chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization. There are two named isoforms.
    • Cellular localization
      Cytoplasm. Endoplasmic reticulum.
    • Database links
    • Alternative names
      • AW552102 antibody
      • c21 LRP antibody
      • C21LRP antibody
      • Chromosome 21 leucine rich protein antibody
      • Down syndrome critical region gene 2 antibody
      • Down syndrome critical region protein 2 antibody
      • Down syndrome critical region protein 2 homolog antibody
      • DSCR2 antibody
      • leucine rich protein C21 LRP antibody
      • LRPC21 antibody
      • PAC-1 antibody
      • PAC1 antibody
      • proteasome (prosome, macropain) assembly chaperone 1 antibody
      • proteasome assembling chaperone 1 antibody
      • Proteasome assembly chaperone 1 antibody
      see all

    Images

    • All lanes : Anti-PSMG1 antibody [EPR10223] (ab167396) at 1/10000 dilution

      Lane 1 : U-87 MG cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 33 kDa
    • Detection of PSMG1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab167396 at 1/10 dilution.
    • Immunofluorescent analysis of HepG2 cells, labeling PSMG1 with ab167396 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSMG1 with ab167396 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeing PSMG1 with ab167396 at 1/250 dilution.

    References

    ab167396 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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