Overview

  • Product nameAnti-Psoriasin antibody [47C1068]
    See all Psoriasin primary antibodies
  • Description
    Mouse monoclonal [47C1068] to Psoriasin
  • Tested applicationsSuitable for: IP, ICC/IF, Flow Cyt, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • Confluent MCF10A cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab13680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 12.1 kDa).
IHC-Fr Use at an assay dependent concentration. (PMID 18606705).
IHC-P Use at an assay dependent concentration.

Target

  • Tissue specificityFetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients.
  • Sequence similaritiesBelongs to the S-101 family.
    Contains 2 EF-hand domains.
  • Cellular localizationCytoplasm. Secreted. Secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • HID 5 antibody
    • Protein S100 A7 antibody
    • Protein S100-A7 antibody
    • PSOR 1 antibody
    • PSOR1 antibody
    • Psoriasin 1 antibody
    • Psoriasin antibody
    • Psoriasin1 antibody
    • S100 Calcium binding protein A7 antibody
    • S100 calcium-binding protein A7 antibody
    • S100A7 antibody
    • S100A7c antibody
    • S10A7_HUMAN antibody
    see all

Anti-Psoriasin antibody [47C1068] images



  • Predicted band size : 12.1 kDa


    Western blot analysis of Psoriasin in confluent MCF10A cells using ab13680 at 1 µg/ml.
  • ICC/IF image of ab13680 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13680, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of Human nasal epithelium, staining Psoriasin with ab13680 at 1/100 dilution. Antigen retrieval was performed in a microwave oven in target retrieval solution (pH 6).

References for Anti-Psoriasin antibody [47C1068] (ab13680)

This product has been referenced in:
  • Qi Z  et al. The Characteristics and Function of S100A7 Induction in Squamous Cell Carcinoma: Heterogeneity, Promotion of Cell Proliferation and Suppression of Differentiation. PLoS One 10:e0128887 (2015). WB ; Human . Read more (PubMed: 26053695) »
  • Gschwandtner M  et al. Fetal Human Keratinocytes Produce Large Amounts of Antimicrobial Peptides: Involvement of Histone-Methylation Processes. J Invest Dermatol N/A:N/A (2014). Read more (PubMed: 24694903) »

See all 10 Publications for this product

Product Wall

Es freut mich zu hören, dass Sie unser Angebot unseren ab13680 Anti-Psoriasin [47C1068] Antikörper in der Maus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Si...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Trititon X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 17 2012

Thank you for contacting Abcam. The epitope region of ab13680 has not been mapped and so I cannot inform you as to where the antibody actually binds. As for making an immunoaffinity column

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (corneal epithelial cells)
Specification corneal epithelial cells
Preparation Cell harvesting/tissue preparation method: Human SV40-transformed immortalised corneal epithelial cell line
Sample buffer: Optimized culture medium containing Epilife (Cascade Biologics, Mansfield, U.K.)
Fixation Formaldehyde
Permeabilization Yes - 0.5% Tween 20
Gating Strategy around 90% of middle area of cell population
Username

Abcam user community

Verified customer

Submitted Nov 30 2010

Thank you for getting back in touch with and for providing me with further details. The difficulties that you have been having are clearly lot related problems. I would like to offer you a free of charge replacement vial of a different lot. However,...

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Thank you for your enquiry. I am sorry to hear that you have had variable results using this antibody. I have read your questionaire and I have a few comments. I am most interested in the variability that you have been observing. 1. Can you ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"