Anti-Psoriasin antibody [47C1068] (ab13680)
Key features and details
- Mouse monoclonal [47C1068] to Psoriasin
- Suitable for: IP, ICC/IF, Flow Cyt, IHC-Fr, Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Psoriasin antibody [47C1068]
See all Psoriasin primary antibodies -
Description
Mouse monoclonal [47C1068] to Psoriasin -
Host species
Mouse -
Tested applications
Suitable for: IP, ICC/IF, Flow Cyt, IHC-Fr, Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Psoriasin.
Database link: P31151 -
Positive control
- Confluent MCF10A cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody is affinity purified. -
Clonality
Monoclonal -
Clone number
47C1068 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 5 µg/ml.
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr | (1) |
Use at an assay dependent concentration.
(PMID 18606705). |
Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 12.1 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Use at an assay dependent concentration. (PMID 18606705). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 12.1 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Tissue specificity
Fetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients. -
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. Secreted. Secreted by a non-classical secretory pathway. - Information by UniProt
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Database links
- Entrez Gene: 6278 Human
- Omim: 600353 Human
- SwissProt: P31151 Human
- Unigene: 112408 Human
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Alternative names
- HID 5 antibody
- Protein S100 A7 antibody
- Protein S100-A7 antibody
see all
Images
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All lanes : Anti-Psoriasin antibody [47C1068] (ab13680) at 1 µg/ml
Lane 1 : MCF10A cell lysate
Lane 2 : MCF7 cell lysate
Predicted band size: 12.1 kDa -
Immunohistochemistry analysis of human esophageal squamous cell carcinoma (SCC) staining psoriasin with ab13680 in 5 µg/mL concentration.
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Immunohistochemistry analysis of formalin-fixed paraffin-embedded human skin tissue after heat-induced antigen retrieval, staining psoriasin with ab13680 in 10 µg/mL concentration.
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Western blot analysis of Psoriasin in confluent MCF10A cells using ab13680 at 1
Western blot analysis of Psoriasin in confluent MCF10A cells using ab13680 at 1 µg/ml. g/ml.µ -
ICC/IF image of ab13680 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13680, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab13680 has been referenced in 25 publications.
- Jung KL et al. Single-cell analysis of Kaposi's sarcoma-associated herpesvirus infection in three-dimensional air-liquid interface culture model. PLoS Pathog 18:e1010775 (2022). PubMed: 35976902
- Luo M et al. Weighted gene coexpression network and experimental analyses identify lncRNA SPRR2C as a regulator of the IL-22-stimulated HaCaT cell phenotype through the miR-330/STAT1/S100A7 axis. Cell Death Dis 12:86 (2021). PubMed: 33452236
- Nguyen T et al. Effective inhibition of Th17/Th22 pathway in 2D and 3D human models of psoriasis by Celastrol enriched plant cell culture extract. J Eur Acad Dermatol Venereol 34 Suppl 6:3-9 (2020). PubMed: 32783265
- Shi ZR et al. Decrease of galectin-3 in keratinocytes: A potential diagnostic marker and a critical contributor to the pathogenesis of psoriasis. J Autoimmun 89:30-40 (2018). WB ; Human . PubMed: 29167025
- Rangaraj A et al. Molecular and cellular impact of Psoriasin (S100A7) on the healing of human wounds. Exp Ther Med 13:2151-2160 (2017). PubMed: 28565822