Overview

  • Product nameAnti-Psoriasin antibody [47C1068]
    See all Psoriasin primary antibodies
  • Description
    Mouse monoclonal [47C1068] to Psoriasin
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant psoriasin/HID-5 protein.

  • Positive control
    • MCF10A cells

Properties

Applications

Our Abpromise guarantee covers the use of ab45091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Tissue specificityFetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients.
  • Sequence similaritiesBelongs to the S-101 family.
    Contains 2 EF-hand domains.
  • Cellular localizationCytoplasm. Secreted. Secreted by a non-classical secretory pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • HID 5 antibody
    • Protein S100 A7 antibody
    • Protein S100-A7 antibody
    • PSOR 1 antibody
    • PSOR1 antibody
    • Psoriasin 1 antibody
    • Psoriasin antibody
    • Psoriasin1 antibody
    • S100 Calcium binding protein A7 antibody
    • S100 calcium-binding protein A7 antibody
    • S100A7 antibody
    • S100A7c antibody
    • S10A7_HUMAN antibody
    see all

Anti-Psoriasin antibody [47C1068] images

  • Anti-Psoriasin antibody [47C1068] (ab45091) at 1 µg/ml + MCF10A cells

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
  • ab45091 staining ductal carcinoma in situ by immunohistochemistry, formalin-fixed, paraffin-embedded sections. Shown are successive sections of H&E staining, a control monoclonal antibody, and ab45091
  • Overlay histogram showing A431 cells stained with ab45091 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45091, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Psoriasin antibody [47C1068] (ab45091)

This product has been referenced in:
  • Boniface K  et al. IL-22 inhibits epidermal differentiation and induces proinflammatory gene expression and migration of human keratinocytes. J Immunol 174:3695-702 (2005). Read more (PubMed: 15749908) »
  • Martinsson H  et al. Expression patterns of S100A7 (psoriasin) and S100A9 (calgranulin-B) in keratinocyte differentiation. Exp Dermatol 14:161-8 (2005). Read more (PubMed: 15740587) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"