Overview

  • Product name
  • Description
    Rabbit polyclonal to PSPC1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Chimpanzee, Chinese hamster, Bat, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 245-275 of Human PSPC1 (NP_001035879.1).

  • Positive control
    • HeLa, 293T or Jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.
  • Tissue specificity
    Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
  • Sequence similarities
    Belongs to the PSPC family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
  • Information by UniProt
  • Database links
  • Alternative names
    • Paraspeckle component 1 antibody
    • Paraspeckle protein 1 antibody
    • PSP1 antibody
    • Pspc1 antibody
    • PSPC1_HUMAN antibody
    see all

Images

  • All lanes : Anti-PSPC1 antibody (ab115184) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 59 kDa


    Exposure time : 3 minutes
  • Samples: whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded).
    Antibodies: ab115184 used for WB at 0.4 µg/ml and used for IP at 6 µg/mg lysate.
    Detection: chemiluminescence with exposure time of 30 seconds (B).

References

This product has been referenced in:
  • Wang J  et al. RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs. J Clin Invest 127:987-1004 (2017). Read more (PubMed: 28192372) »

See 1 Publication for this product

Customer reviews and Q&As

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (P19 Embryonal carcinoma cells)
Specification
P19 Embryonal carcinoma cells
Fixative
Formaldehyde
Permeabilization
Yes - PBS with 0.5% Triton-X100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mrs. Filomena Ricciardi

Verified customer

Submitted May 25 2012

DISCOUNT CODES:

ab115184: x


ab116103: y


Ablaufdatum jeweils: dd mm 2012

Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper der IF zu testen, annehmen möchten. Wie besprochen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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