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Rabbit polyclonal to PTBP1
Predicted to work with:
Mouse, Rat, Chicken, Cow, Pig, Xenopus tropicalis
Recombinant fragment corresponding to a region within amino acids 293-528 of Human PTBP1 (UniProt ID: P26599).
293T, A431, HeLa and HepG2 whole cell lysate. Human AGS xenograft tissue.
Keep as concentrated solution.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
Contains 4 RRM (RNA recognition motif) domains.
Information by UniProt
57 kDa RNA binding protein PPTB 1 antibody
57 kDa RNA-binding protein PPTB-1 antibody
Heterogeneous nuclear ribonucleoprotein I antibody
Western blot - Anti-PTBP1 antibody (ab154887)
All lanes : Anti-PTBP1 antibody (ab154887) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lane 3 : HeLa whole cell lysate Lane 4 : HepG2 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 57 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP1 antibody (ab154887)
Immunohistochemical analysis of paraffin embedded Human AGS xenograft tissue, labeling PTBP1 using ab154887 at 1/500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"