Overview

  • Product name
  • Description
    Rabbit polyclonal to PTBP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 305-500 of Human PTBP2 (UniProt: Q9UKA9).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 whole cell lysates; Human Cal27 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA.
  • Tissue specificity
    Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues.
  • Sequence similarities
    Contains 4 RRM (RNA recognition motif) domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • brPTB antibody
    • FLJ34897 antibody
    • MIBP antibody
    • Neural polypyrimidine tract binding protein antibody
    • Neural polypyrimidine tract-binding protein antibody
    • Neurally enriched homolog of PTB antibody
    • Neurally-enriched homolog of PTB antibody
    • nPTB 5 antibody
    • nPTB 6 antibody
    • nPTB 7 antibody
    • nPTB 8 antibody
    • nPTB antibody
    • nPTB5 antibody
    • nPTB6 antibody
    • nPTB7 antibody
    • nPTB8 antibody
    • Polypyrimidine tract binding protein 2 antibody
    • Polypyrimidine tract-binding protein 2 antibody
    • PTB antibody
    • PTB like antibody
    • PTB like protein antibody
    • PTB-like protein antibody
    • PTBLP antibody
    • PTBP 2 antibody
    • Ptbp2 antibody
    • PTBP2_HUMAN antibody
    • Splicing regulator antibody
    see all

Images

  • Anti-PTBP2 antibody (ab154920) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue, labeling PTBP2 with ab154920 at 1/500 dilution.

References

ab154920 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154920.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up