Overview

  • Product nameAnti-PTBP2 antibody
    See all PTBP2 primary antibodies
  • Description
    Mouse monoclonal to PTBP2
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) with GST tag

Properties

Applications

Our Abpromise guarantee covers the use of ab57619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 58 kDa.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionRNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA.
  • Tissue specificityMainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues.
  • Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • brPTB antibody
    • FLJ34897 antibody
    • MIBP antibody
    • Neural polypyrimidine tract binding protein antibody
    • Neural polypyrimidine tract-binding protein antibody
    • Neurally enriched homolog of PTB antibody
    • Neurally-enriched homolog of PTB antibody
    • nPTB 5 antibody
    • nPTB 6 antibody
    • nPTB 7 antibody
    • nPTB 8 antibody
    • nPTB antibody
    • nPTB5 antibody
    • nPTB6 antibody
    • nPTB7 antibody
    • nPTB8 antibody
    • Polypyrimidine tract binding protein 2 antibody
    • Polypyrimidine tract-binding protein 2 antibody
    • PTB antibody
    • PTB like antibody
    • PTB like protein antibody
    • PTB-like protein antibody
    • PTBLP antibody
    • PTBP 2 antibody
    • Ptbp2 antibody
    • PTBP2_HUMAN antibody
    • Splicing regulator antibody
    see all

Anti-PTBP2 antibody images



  • Predicted band size : 58 kDa
    PTBP2 antibody (ab57619) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • Overlay histogram showing SH-SY5Y cells stained with ab57619 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57619, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-PTBP2 antibody (ab57619)

This product has been referenced in:

See 1 Publication for this product

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DISCOUNT CODE: 100%00000


Expiration date: 20. October 2012

I am very pleased to hear you would like to accept our offer and test ab57619 in ICC/IF.

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Thank you very much for your interest in ab57619.

Because we carry over 75,000 products, it isn't feasible for us to keep small sample sizes of our products.

I am happy to confirm that ab57619istested and guaranteed for WB.
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Thank you for your e-mail. We have not tested the cross reactivity between PTBP1 and PTBP2 or other products yet. We feel sorry that at this moment we have no information about their cross reactivity. Please feel free to contact me if I can provide fu...

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Thank you for your enquiry. Unfortunately with regards to the PTBP2 monoclonal antibody ab57619, we have not tested the cross reactivity with other isoforms of PTB, and we do not know what is the epitope recognized by the antibody therefore cannot pred...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"