Overview

  • Product name
    Anti-PTCHD1 antibody
  • Description
    Goat polyclonal to PTCHD1
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-DFQKTSRVSER

    , corresponding to internal sequence amino acids 260-270 of Human PTCHD1 (NP_775766.2).

  • Positive control
    • Human Brain (Cerebellum) lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab109407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 101 kDa).

Target

Images

  • Anti-PTCHD1 antibody (ab109407) at 0.5 µg/ml + Human Brain Cerebellum lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 101 kDa



    An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide.

References

ab109407 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (cerebellum, kidney)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
70 µg
Specification
cerebellum, kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mike Liu

Verified customer

Submitted Apr 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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