Anti-PTF1A antibody [1A2] (ab57257)

Overview

  • Product name
    Anti-PTF1A antibody [1A2]
    See all PTF1A primary antibodies
  • Description
    Mouse monoclonal [1A2] to PTF1A
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human PTF1A aa 250-328.
    Sequence:

    QAQKVIICHRGTRSPSPSDPDYGLPPLAGHSLSWTDEKQLKEQNIIRTAK VWTPEDPRKLNSKSSFNNIENEPPFEFVS


    Database link: Q7RTS3

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab57257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.
IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.

Target

  • Function
    Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells (By similarity). Plays an important role in cerebellar development.
  • Tissue specificity
    Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.
  • Involvement in disease
    Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH) [MIM:609069].
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus. Cytoplasm. In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex.
  • Information by UniProt
  • Database links
  • Form
    PTF1A is a pancreas specific transcription factor. Mammalian studies have implicated important roles for the basic helix-loop-helix transcription factor PTF1A-p48 in the development of both exocrine and endocrine pancreas.
  • Alternative names
    • bHLH transcription factor p48 antibody
    • bHLHa29 antibody
    • Class A basic helix-loop-helix protein 29 antibody
    • Class II bHLH protein PTF1A antibody
    • Exocrine pancreas specific transcription factor p48 antibody
    • p48 DNA binding subunit of transcription factor PTF1 antibody
    • p48 DNA-binding subunit of transcription factor PTF1 antibody
    • PACA antibody
    • PAGEN2 antibody
    • Pancreas specific transcription factor 1a antibody
    • Pancreas transcription factor 1 subunit alpha antibody
    • Pancreas-specific transcription factor 1a antibody
    • PTF 1A antibody
    • PTF1 p48 antibody
    • PTF1-p48 antibody
    • Ptf1a antibody
    • PTF1A_HUMAN antibody
    • PTF1P48 antibody
    see all

Images

  • Western blot against tagged recombinant protein immunogen using ab57257 PTF1A antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
  • PTF1A antibody (ab57257) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human esophagus.
  • Immunofluorescence of ab57257 on HeLa cell at the concentration of 10 ug/ml.

References

ab57257 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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What I meant by different targets against the same antibody, is that we sometimes have multiply antibodies that recognize the same target in our catalogue.

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I am sorry that you are having some technical issues with a few of our antibodies. These antibodiesare covered under our Abpromise for six months andareguaranteed to work in IHC-P on human samples . If we canno...

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