Recombinant
RabMAb

Anti-PTF1A antibody [EPR19011] (ab182398)

Overview

  • Product name
    Anti-PTF1A antibody [EPR19011]
    See all PTF1A primary antibodies
  • Description
    Rabbit monoclonal [EPR19011] to PTF1A
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Mouse PTF1A aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9QX98

  • Positive control
    • WB: Human fetal heart and fetal kidney lysates; BxPC-3, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Rat heart and pancreas lysates; Mouse pancreas lysate. IP: NIH/3T3 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab182398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
WB 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa).

Target

  • Function
    Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells (By similarity). Plays an important role in cerebellar development.
  • Tissue specificity
    Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.
  • Involvement in disease
    Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH) [MIM:609069].
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus. Cytoplasm. In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex.
  • Information by UniProt
  • Database links
  • Form
    PTF1A is a pancreas specific transcription factor. Mammalian studies have implicated important roles for the basic helix-loop-helix transcription factor PTF1A-p48 in the development of both exocrine and endocrine pancreas.
  • Alternative names
    • bHLH transcription factor p48 antibody
    • bHLHa29 antibody
    • Class A basic helix-loop-helix protein 29 antibody
    • Class II bHLH protein PTF1A antibody
    • Exocrine pancreas specific transcription factor p48 antibody
    • p48 DNA binding subunit of transcription factor PTF1 antibody
    • p48 DNA-binding subunit of transcription factor PTF1 antibody
    • PACA antibody
    • PAGEN2 antibody
    • Pancreas specific transcription factor 1a antibody
    • Pancreas transcription factor 1 subunit alpha antibody
    • Pancreas-specific transcription factor 1a antibody
    • PTF 1A antibody
    • PTF1 p48 antibody
    • PTF1-p48 antibody
    • Ptf1a antibody
    • PTF1A_HUMAN antibody
    • PTF1P48 antibody
    see all

Images

  • All lanes : Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution

    Predicted band size : 35 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight  observed is consistent with what has been described in the literature (PMID: 18347078).

  • All lanes : Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution

    Lane 1 : Rat heart lysate at 20 µg
    Lane 2 : Rat pancreas lysate at 20 µg
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 35 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lane 3, 4, 5 and 6: 10 seconds.

  • Anti-PTF1A antibody [EPR19011] (ab182398) at 1/5000 dilution + Mouse pancreas lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 35 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • PTF1A was immunoprecipitated from 1 mg of NIH/3T3 (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab182398 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab182398 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).

    Lane 2: ab182398 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182398 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

ab182398 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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