The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 µg/ml.
Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
RelevanceParathyroid related protein (PTHLH), is a polypeptide hormone produced by almost every tissue of the body and is closely related to parathyroid hormone (PTH). It signals through its receptor, PTHR1, regulating endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. The receptor for this hormone (PTHRP) is responsible for most cases of humoral hypercalcemia of malignancy.
Cellular localizationCytoplasmic, Nuclear and Secreted
Parathyroid hormon like hormone isoform 2 preproprotein antibody
Parathyroid hormone like hormone antibody
Parathyroid hormone like hormone isoform 1 preproprotein antibody
Parathyroid hormone like protein antibody
Parathyroid hormone like related protein antibody
Parathyroid hormone related protein antibody
Parathyroid like protein antibody
PTH related protein antibody
PTH rP antibody
Anti-PTHLH antibody images
Flow Cytometry - Anti-PTHLH antibody (ab55631)
Overlay histogram showing HepG2 cells stained with ab55631 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55631, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
IHC-P - PTHLH antibody (ab55631)
PTHLH antibody (ab55631) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
Western blot - PTHLH antibody (ab55631)
Western blot against tagged recombinant protein immunogen using ab55631 PTHLH antibody at 1ug/ml. Predicted band size of immunogen is 45 kDa
References for Anti-PTHLH antibody (ab55631)
has not yet been referenced specifically in any publications.
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