Overview

  • Product name
  • Description
    Mouse polyclonal to PTIP
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human PTIP1 (amino acids 1-757; AAH33781.1).

  • Positive control
    • PTIP transfected 293T cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab168502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires RNF8 and UBE2N. Recruits TP53BP1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with TP53BP1 phosphorylated by ATM at 'Ser-25'. Together with TP53BP1 regulates ATM association. Recruits PA1 to sites of DNA damage and the PA1:PAXIP1 complex is required for cell survival in response to DNA damage; the function is probbaly independent of MLL-containing histone methyltransferase (HMT) complexes. Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and RAD51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as PAX2. Associates with gene promoters that are known to be regulated by MLL2. During immunoglobulin class switching in activated B cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL-containing HMT complexes.
  • Sequence similarities
    Contains 6 BRCT domains.
  • Domain
    The BRCT 5 and 6 domains function as a single module and are necessary and sufficient for in vitro phospho-specific binding (substrates phosphorylated by the kinases ataxia telangiectasia-mutated (ATM), ataxia telangiectasia and RAD3-related (ATR) in response to gamma irradiation). In contrast, in vivo two pairs of BRCT domains (3-6) bind to phosphorylated TP53BP1 much more efficiently.
  • Cellular localization
    Nucleus matrix. Localizes to DNA damage foci upon ionizing radiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAGF 28 antibody
    • CAGF 29 antibody
    • CAGF28 antibody
    • CAGF29 antibody
    • FLJ41049 antibody
    • PACIP 1 antibody
    • PACIP1 antibody
    • PAX interacting (with transcription activation domain) protein 1 antibody
    • PAX interacting protein 1 antibody
    • PAX transactivation activation domain-interacting protein antibody
    • PAX transcription activation domain interacting protein 1 like antibody
    • PAX-interacting protein 1 antibody
    • PAXI1_HUMAN antibody
    • PAXIP 1 antibody
    • PAXIP 1L antibody
    • paxip1 antibody
    • PAXIP1 protein antibody
    • PAXIP1L antibody
    • Protein encoded by CAG trinucleotide repeats antibody
    • PTIP antibody
    • TNRC 2 antibody
    • TNRC2 antibody
    see all

Images

  • All lanes : Anti-PTIP antibody (ab168502) at 1 µg/ml

    Lane 1 : PTIP1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 83 kDa

  • Immunofluorescent analysis of HeLa cells labeling PTIP1 with ab168502 at 10 µg/ml.

References

ab168502 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon adenocarcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Comercial neutral pH buffer
Permeabilization
Yes - Tween 0,1% TBS
Specification
Colon adenocarcinoma
Blocking step
Casein as blocking agent for 5 minute(s) · Concentration: 0.4% · Temperature: 25°C
Fixative
Formaldehyde
Username

Giuliana De Gregoris

Verified customer

Submitted Nov 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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