Overview

  • Product name
  • Description
    Rabbit polyclonal to PTOP
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 150-200 of Human PTOP (NP_001075955.1 (GeneID 65057)).

  • Positive control
    • HeLa and Jurkat whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab112050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity. Plays a role in shelterin complex assembly. May play a role in organogenesis.
  • Cellular localization
    Nucleus. Chromosome > telomere.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acd antibody
    • ACD_HUMAN antibody
    • Adrenocortical dysplasia homolog antibody
    • Adrenocortical dysplasia protein homolog antibody
    • PIP1 antibody
    • POT1 and TIN2 organizing protein antibody
    • POT1 and TIN2-interacting protein antibody
    • TIN2 interacting protein 1 antibody
    • TINT1 antibody
    • TPP1 antibody
    see all

Images

  • All lanes : Anti-PTOP antibody (ab112050) at 0.4 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 58 kDa


    Exposure time : 3 minutes
  • ab112050 at 1 µg/ml staining PDE3A in HeLa cell lysate immunoprecipitated using ab112050 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: controle IgG. Predicted band size : 58 kDa Exposure time: 30 seconds

References

ab112050 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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