• Product nameAnti-PTOP antibody
    See all PTOP primary antibodies
  • Description
    Mouse monoclonal to PTOP
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-545 of Human PTOP



Our Abpromise guarantee covers the use of ab57595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 2 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionComponent of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity. Plays a role in shelterin complex assembly. May play a role in organogenesis.
  • Cellular localizationNucleus. Chromosome > telomere.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acd antibody
    • ACD_HUMAN antibody
    • Adrenocortical dysplasia homolog antibody
    • Adrenocortical dysplasia protein homolog antibody
    • PIP1 antibody
    • POT1 and TIN2 organizing protein antibody
    • POT1 and TIN2-interacting protein antibody
    • TIN2 interacting protein 1 antibody
    • TINT1 antibody
    • TPP1 antibody
    see all

Anti-PTOP antibody images

  • PTOP antibody (ab57595) used in immunofluorescence at 10ug/ml on HeLa cells.
  • PTOP antibody (ab57595) used in immunohistochemistry at 2ug/ml on formalin fixed and paraffin embedded human heart tissue.

  • Predicted band size : 57 kDa
    PTOP antibody (ab57595) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab57595 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57595, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-PTOP antibody (ab57595)

This product has been referenced in:
  • Li Z  et al. PTOP and TRF1 help enhance the radio resistance in breast cancer cell. Cancer Cell Int 14:7 (2014). WB ; Human . Read more (PubMed: 24460895) »
  • Chen J  et al. Sirtuin 1 is upregulated in a subset of hepatocellular carcinomas where it is essential for telomere maintenance and tumor cell growth. Cancer Res 71:4138-49 (2011). WB ; Human . Read more (PubMed: 21527554) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. This was one of the hard to make product which has been discontinued by our collaborator. They do not produce this antibody anymore so we were forced to unpublish the product. We apologize for any inconvenience this...

Read More