Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP alpha antibody (ab77792)Image from Khoontawad J et al., PLoS One. 2012;7(9):e45460. doi: 10.1371/journal.pone.0045460. Epub 2012 Sep 18. Fig 5.; doi:10.1371/journal.pone.0045460; September 18, 2012, PLoS ONE 7(9): e45460.
Immunohistochemical analysis of Human liver tissue, staining PTP alpha with ab77792.
Antigen retrieval was performed via heat mediation in a citrate buffer (pH 6) before blocking in %5 fetal bovine serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. Staining was detected with DAB.